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pCMV HA hRB delta CDK + T356

Plasmid

#58911

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T356 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S780

Plasmid

#58915

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S780 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S788

Plasmid

#58916

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S788 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S807

Plasmid

#58918

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S807 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T821

Plasmid

#58920

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T821 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 RL3Am

Plasmid

#109364

Purpose

Human rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Human, Branchiostoma belcheri)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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cftr_R (OZ518)

Plasmid

#27193

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting cftr (cftr Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
RB-409-815-pGex2T

Plasmid

#21784

Depositor

William Kaelin

Article

Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 409-815 of wt Rb …

Available Since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 WT

Plasmid

#184627

Purpose

Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)

Expression

Mammalian

Mutation

no mutation

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1 KBTBD4 WT

Plasmid

#184624

Purpose

Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

no mutation

Promoter

TRE promoter, Tet ON

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171596

Purpose

Ectodomain deleted negative control ACE2 from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

Plasmid

#91882

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Suz12 (Suz12 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pCR-Blunt II-TOPO-PTPRD

Plasmid

#29705

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta (PTPRD Human)

Use

Cloning vector

Mutation

none

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
cmv 16 E7 del PTLHE

Plasmid

#13688

Depositor

Karl Munger

Article

Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

Insert

HPV 16 E7 del PTLHE (E7 Human)

Expression

Mammalian

Mutation

del aa 6-10

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pLV-PLXNB2

Plasmid

#86237

Purpose

Lentivirus for expression of human Plexin-B2

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

full length PLXNB2, CRISPR resistant

Promoter

human PGK promoter

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGLO-Bmpr2-Δ17

Plasmid

#49380

Purpose

Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.

Depositor

Martine Roussel

Article

Murphy et al Cancer Res. 2013 Oct 21.

Insert

Bmpr2 (BMPR2 Human, Mouse)

Use

Luciferase

Mutation

changed nucleotides AC to TG at bp 7347-7348

Promoter

Human phosphoglycerate kinase (PGK) promoter

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTorPE-GEX-GECO1

Plasmid

#32464

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEX-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

Plasmid

#171801

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-FLAG-hgp100-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pIE-TGFB2-H6

Plasmid

#128500

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Tags

His tag (6x)

Expression

Insect

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-EGFP

Plasmid

#48349

Purpose

Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EGFP

Use

Gateway Cloning

Mutation

Aminoacid 40 is valine in our sequence, while it …

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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