We narrowed to 6,082 results for: pcDNA 3.1

1. pcDNA3.1+_Lambda-N-HA-Peptide

   Plasmid

   #92005

   Purpose

   Expresses Lambda-N-HA peptide

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   Lambda-N-HA peptide
   

   Tags

   Lambda-N-HA

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/VChR1-EYFP

   Plasmid

   #20955

   Purpose

   Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

   Insert

   volvox channelrhodopsin-1
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-30nmEML-mRFP

   Plasmid

   #231179

   Purpose

   Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   30nmEML-mRFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-15nmEML-mRFP

   Plasmid

   #231177

   Purpose

   Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   15nmEML-mRFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MS2-Tet1-CD

   Plasmid

   #83341

   Purpose

   To introduce MS2 coat protein fused Tet1-CD

   Depositor

   Ronggui Hu

   Article

   Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

   Insert

   MS2-Tet1-CD
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-P25C-GFP

   Plasmid

   #1343

   Depositor

   Li-Huei Tsai

   Insert

   P25 (CDK5R1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1.Gαi1-LgB91

   Plasmid

   #134342

   Purpose

   Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαi1-LgB91 (GNAI1 Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-300(HAT-)

   Plasmid

   #23254

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Expression

   Mammalian

   Mutation

   H1415A E1423A Y1424A L1428S Y1430A H1434A

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Gαq*-BERKY3

   Plasmid

   #158217

   Purpose

   BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3.1-Bsd-mEGFP

    Plasmid

    #214369

    Purpose

    Expression of the monomeric Green Fluorescent Protein

    Depositor

    Bertrand Collet

    Insert

    mEGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRP4-Flag/pCDNA3.1

    Plasmid

    #197291

    Purpose

    Plasmid expressing an antigen targeting LRP4 (extracellular)

    Depositor

    Steve Burden

    Insert

    LRP4 (Lrp4 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-FLAG-RGS14

    Plasmid

    #236567

    Purpose

    Expresses Flag-RGS14 in mammalian cells

    Depositor

    John Hepler

    Article

    Friedman et al J Biol Chem. 2022 May;298(5):101836. doi

    Insert

    Homo sapiens regulator of G-protein signaling 14 (RGS14 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. RGS-6xHis-pcDNA3.1-

    Plasmid

    #52534

    Purpose

    enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Type

    Empty backbone

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Rho*-BERKY3

    Plasmid

    #158220

    Purpose

    BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 19, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

    Plasmid

    #131033

    Purpose

    Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRA (Tcra Mouse)
    TCRB (Tcrb Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV/T7

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-VEGFR3-V5/HIS

    Plasmid

    #205618

    Purpose

    Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    VEGFR3 receptor tyrosine kinase (FLT4 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    T28M, S455R- Please see depositor comment

    Promoter

    CMV

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)GαoA-91-NLuc

    Plasmid

    #207151

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAO
    

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)G

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)GαoB-91-NLuc

    Plasmid

    #207152

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAO
    

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)G

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 3xFlag IFIT3

    Plasmid

    #53553

    Purpose

    mammalian expression of IFIT3

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT3 (IFIT3 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1(+)-FluoSTEP-AKAR

    Plasmid

    #181844

    Purpose

    Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-AKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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