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pcDNA3.1(+)-ExRai-AKAR2(T/A)

Plasmid

#161754

Purpose

Negative-control mutant for ExRai-AKAR2 biosensor.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2(T/A)

Use

Tags

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HisC EXO1b

Plasmid

#68268

Purpose

Mammalian expression of human EXO1

Depositor

Stefano Ferrari

Article

El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

Insert

EXO1 (EXO1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

b form, codons 1-846. Please see depositor commen…

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Bsd-mEGFP

Plasmid

#214369

Purpose

Expression of the monomeric Green Fluorescent Protein

Depositor

Bertrand Collet

Insert

mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-EGFP_AB

Plasmid

#148304

Purpose

Mammalian Expression vector

Depositor

Elisa Izaurralde

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 E

Plasmid

#158080

Purpose

Expresses SARS-CoV-2 envelope protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 envelope (E )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DDR1-V5/HIS

Plasmid

#201100

Purpose

expression of human DDR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human DDR1 receptor tyrosine kinase, full length, wildtype (DDR1 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1

Plasmid

#128768

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Dead-Laconic/pcDNA3.1(-)

Plasmid

#118627

Purpose

To express in mammalian cells a lactate FRET sensor negative control.

Depositor

Luis Felipe Barros

Article

Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

Insert

Dead-Laconic

Use

Tags

Expression

Mammalian

Mutation

Change Histidine 151 to Aspartic Acid of Escheric…

Promoter

CMV Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cry2FL-VP64

Plasmid

#60554

Purpose

Expresses Cry2FL-VP64 in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

Cry2FL-VP64

Use

Tags

FLAG, HA tag, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 AK3 Hyg

Plasmid

#37539

Depositor

David Sidransky

Article

Chang et al PLoS One. 2011;6(6):e20806. Epub 2011 Ju

Insert

AK3 (AK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-DDR2-V5/HIS

Plasmid

#201101

Purpose

expression of human DDR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human DDR2 receptor tyrosine kinase, full length, wildtype (DDR2 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNBAS_AA

Plasmid

#148236

Purpose

Mammalian Expression of HsNBAS

Depositor

Elisa Izaurralde

Insert

HsNBAS (NBAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-V5/HIS

Plasmid

#201987

Purpose

expression of human PDGFRA receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA receptor tyrosine kinase (PDGFRA Human)

Use

Tags

V5, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-RBM24

Plasmid

#208052

Purpose

Expressing N-ter Flag tagged human RBM24 (ENST00000379052) in mammalian cells

Depositor

Quanfu Ma

Insert

human RBM24 (ENST00000379052) coding region (RBM24 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-mSTING (N153S)

Plasmid

#214158

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (N153S) (Sting1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

N153S

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_C223S USP7

Plasmid

#131243

Purpose

Mammalian expression of catalytically inactive Myc-tagged USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Contains a point mutation that converts USP7 cyst…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-StrepHA-SUMO2

Plasmid

#66867

Purpose

Mammalian expression of SUMO2 with a strep and an HA tag

Depositor

Ying Liu

Article

Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

Insert

SUMO2 (SUMO2 Human)

Use

Tags

HA and Strep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc TSC1

Plasmid

#12133

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC1 (TSC1 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

Plasmid

#159128

Purpose

expresses KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

KEAP1 (KEAP1 Human)

Use

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαi1-LgB91

Plasmid

#134342

Purpose

Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαi1-LgB91 (GNAI1 Human)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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