We narrowed to 2,579 results for: pcas

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pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

Plasmid

#185915

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8.20m-nSpCas9-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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p23-NES-PspCas13b-msfGFP-NES-Flag

Plasmid

#165071

Purpose

overexpression of PspCas13b in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

PspCas13b

Use

Lentiviral

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9_soc_crRNA

Plasmid

#257705

Purpose

Bacterial expression plasmid encoding Cas9 nuclease, tracrRNA, and crRNA targeting the soc gene of T4 phage

Depositor

Juhong Chen

Article

He et al Anal Chem. 2024 Nov 12;96(45):18301-1831

Insert

SpCas9

Use

CRISPR

Expression

Bacterial

Available Since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pE-SUMO_PspCas13b

Plasmid

#252295

Purpose

Expresses PspCas13b (WT) in bacterial cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

PspCas13b (WT)

Expression

Bacterial

Mutation

WT

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9_antiBFP

Plasmid

#220922

Purpose

Expresses Cas9 with a BFP targeting crRNA for use in targeting the BFP plasmid in a cell based assay

Depositor

Ryan Jackson

Article

Walters et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Insert

pCas9

Expression

Bacterial

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9_antiGFP

Plasmid

#220923

Purpose

Expresses Cas9 with a GFP targeting crRNA for use in targeting the GFP plasmid in a cell based assay

Depositor

Ryan Jackson

Article

Walters et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Insert

pCas9

Expression

Bacterial

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9_antiOFP

Plasmid

#220924

Purpose

Expresses Cas9 with a OFP targeting crRNA for use in targeting the OFP plasmid in a cell based assay

Depositor

Ryan Jackson

Article

Walters et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Insert

pCas9

Expression

Bacterial

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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p2T_CAG_SpCas9PE2_5plinker_BlastR

Plasmid

#173066

Purpose

Expresses PE2

Depositor

Richard Sherwood

Article

Velimirovic et al Nat Commun. 2022 Jun 18;13(1):3512. doi:

Insert

5plinker

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pPCaspase

Plasmid

#214042

Purpose

Bacterial expression plasmid for PCaspase from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCas4_1_Δ2_VA

Plasmid

#186439

Purpose

Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCasΔ4_1_2_VA

Plasmid

#186440

Purpose

Encodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas1 and Cas2

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12(PI)

Plasmid

#186448

Purpose

Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12a (PI)

Use

CRISPR

Mutation

K613A, K617A

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12b

Plasmid

#186449

Purpose

Encodes Cas12b from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12b(PI)

Plasmid

#186451

Purpose

Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b (PI)

Use

CRISPR

Mutation

R122A, G143P

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF

Plasmid

#177267

Purpose

CRISPR vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar1_ccC

Plasmid

#158121

Purpose

deletion mAdar1

Depositor

Emanuela Felley-Bosco

Insert

Adar1 (Adar Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar2_ccC

Plasmid

#158117

Purpose

deletion mADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar2_ccA

Plasmid

#158118

Purpose

deletion mADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar2_ccB

Plasmid

#158119

Purpose

deletion mADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFB_ROSA26_SpCas9_gRNAs

Plasmid

#174703

Purpose

Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

Depositor

Beverly L. Davidson

Article

Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

Insert

SpCas9 dual gRNAs

Use

AAV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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