Addgene

We narrowed to 1,598 results for: pfas

Showing: 681 - 700 of 1598 results

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-3HA-KanMX6

Plasmid

#228020

Purpose

For TurboID-FRB integration; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-3HA-HIS3MX6

Plasmid

#228021

Purpose

For TurboID-FRB integration; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-3HA-HIS3MX6

Plasmid

#228025

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-3HA-KanMX6

Plasmid

#228024

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-rDPP9

Plasmid

#217326

Purpose

Purification of rat DPP9 from insect cells

Depositor

Yifan Wang

Insert

DPP9

Use

Tags

His-TEV

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

Plasmid

#216378

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

HA signal sequence-FLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

Plasmid

#198037

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

E696K

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-TEV-mCherry-TBK1

Plasmid

#198033

Purpose

Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV and mCherry

Expression

Insect

Mutation

E696K

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB MBP-SARM1 A600G

Plasmid

#195764

Purpose

Purification of SARM1 from insect cells

Depositor

Hao Wu

Insert

SARM1 (SARM1 Human)

Use

Tags

HIS-MBP-TEV

Expression

Insect

Mutation

A600G

Promoter

polyhedrin

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-M13-6His-KIF1C_R169W-GFP

Plasmid

#185979

Purpose

Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.

Depositor

Anne Straube

Article

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Use

Tags

6xHis and eGFP

Expression

Insect

Mutation

R169W - mutation causing HSP (SPG58)

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-M13-6His-KIF1C_P176L-GFP

Plasmid

#185978

Purpose

Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.

Depositor

Anne Straube

Article

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Use

Tags

6xHis and eGFP

Expression

Insect

Mutation

P176L - mutation causing HSP (SPG58)

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)

Plasmid

#79035

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTF257 (pFA6a-TEV-6xGly-His3MX)

Plasmid

#44068

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

Expression

Mutation

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only

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pTF258 (pFA6a-PSc-6xGly-His3MX)

Plasmid

#44062

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

Expression

Mutation

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only

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pTF261 (pFA6a-TEV-6xGly-HphMX)

Plasmid

#44080

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

Expression

Mutation

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pTF262 (pFA6a-PSc-6xGly-HphMX)

Plasmid

#44074

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

Expression

Mutation

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pTF259 (pFA6a-TEV-6xGly-KanMX)

Plasmid

#44092

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

Expression

Mutation

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-Lckg2a,Y394F,Y505F)

Plasmid

#177894

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Use

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F,Y505F

Promoter

Polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

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pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)

Plasmid

#44083

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

3xFlag, 6xGly linker, ADH1 transcription terminat…

Expression

Mutation

Promoter

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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