Addgene

Showing: 681 - 700 of 1412 results

Results per page:

Sort results by:

pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178574

Purpose

Lentiviral vector for CamKIIα::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-BTLA (Y226F, Y243F, Y257F, Y282F)-mGFP

Plasmid

#180792

Purpose

Expression of human BTLA (Y226F, Y243F, Y257F, Y282F) fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLA (BTLA Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

Y226F, Y243F, Y257F, Y282F

Promoter

SFFV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

Plasmid

#173862

Purpose

Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)

Depositor

Takao Nakata

Article

Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

Insert

AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP

Plasmid

#137153

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

W179F

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178575

Purpose

Lentiviral vector for GFAP::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

GFAP

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

Plasmid

#210605

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional base editor

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

ABE8e-hyperdCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

truncated hsp70

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

Plasmid

#47455

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Use

AAV

Tags

2A_GFP

Expression

Mammalian

Mutation

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

Plasmid

#214877

Purpose

Lentiviral vector encoding RfxCas13d targeting LPT guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crLPT-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A

Plasmid

#74606

Purpose

expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Y101A, Y103A, W120A

Promoter

SFFV

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

Plasmid

#145268

Purpose

Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP

Plasmid

#192609

Purpose

Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS-P2A-tagBFP

Use

Lentiviral and Synthetic Biology

Tags

tagBFP

Expression

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)

Plasmid

#214882

Purpose

Lentiviral vector encoding RfxCas13d targeting PROX guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crPROX-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

Plasmid

#214879

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF2-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SV40-mCherry-SHP1 (SHP2-nSH2, cSH2)

Plasmid

#180823

Purpose

Expression of N-terminal mCherry tagged human SHP1 with its nSH2 and cSH2 replaced by nSH2 and cSH2 of SHP2

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

SHP1 (SHP2-nSH2, cSH2) (PTPN6, PTPN11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SV40

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-HOXD13(DEdel)-IDR-mCherry-CRY2-NLS

Plasmid

#145273

Purpose

Mammalian Expression of Nuclear Opto HOXD13(DEdel) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(D/E del)-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

D/E deleted

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-HOXD13(-15A)-IDR-mCherry-CRY2-NLS

Plasmid

#145272

Purpose

Mammalian Expression of Nuclear Opto HOXD13(-15A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(-15A)-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat deleted

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

Plasmid

#214875

Purpose

Empty lentiviral backbone for guide array cloning

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

Plasmid

#60910

Purpose

dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, and NLS

Expression

Mammalian

Mutation

Promoter

dSV40 Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

Plasmid

#121966

Purpose

Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

scFv-GCN4

Use

Lentiviral

Tags

superfold GFP-GB1-NLS-iLID

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP

Plasmid

#223215

Purpose

Lentiviral vector - mCherry reporter with a BAMH1 site for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

mCherry BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge