Addgene

We narrowed to 6,344 results for: sas

Showing: 681 - 700 of 6344 results

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pSMP-RING1_2

Plasmid

#36356

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

RING1 (RING1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pAG426GPD-ccdB-YFP-yeastSMASh

Plasmid

#68855

Purpose

Expresses SMASh tag fused to YFP at C terminus in yeast

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

YFP-yeastSMASh

Use

Tags

SMASh tag

Expression

Yeast

Mutation

Promoter

GPD

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA CAG

Plasmid

#50927

Purpose

U6 driven sgRNA negative control

Depositor

Scot Wolfe, Rene Maehr

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

negative control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

Plasmid

#64113

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mTwist

Plasmid

#1783

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

Twist (Twist1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2005

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1hWT

Plasmid

#35027

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1 (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-Calreticulin-N-10

Plasmid

#57353

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Calreticulin (CALR Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FUGW-d2GFP-200

Plasmid

#79602

Purpose

FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

d2GFP + miR-200 family targets

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9_sgRNA_expression_in_pBluescript

Plasmid

#122089

Purpose

U6 driven SpCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FRBl2-E

Plasmid

#15289

Depositor

David Spencer

Article

Li et al Gene Ther. 2002 Feb . 9(4):233-44.

Insert

FRAP-rapamycin binding domain (MTOR Human)

Use

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

res. 2025-2113, T2098L

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shFEN

Plasmid

#17921

Depositor

Sheila Stewart

Article

Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

Insert

shFEN (FEN1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-CDC25A

Plasmid

#216511

Purpose

Expresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CDC25A (CDC25A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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NmCas9_sgRNA_expression_in_pBluescript

Plasmid

#122091

Purpose

U6 driven NmCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-epNTR

Plasmid

#62213

Purpose

epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

Depositor

Harold Burgess

Article

Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

Insert

epNTR

Use

Mrna synthesis

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-shNTC

Plasmid

#217638

Purpose

Dox inducible shRNA non targeting control shRNA

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shNTC

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSKB-

Plasmid

#62540

Purpose

Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

Plasmid

#64112

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-Fascin-C-10

Plasmid

#57376

Purpose

Localization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Fascin (FSCN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
EC71100

Plasmid

#154068

Purpose

Level 0 Golden Gate vector, pHvHSP17

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

pHvHSP17

Use

Standard cloning vector used by geneart for synth…

Tags

Expression

Mutation

All BsaI, Esp3I and BpiI restriction sites were r…

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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