19,660 results

1. pDONR223_KRAS_p.G12D

   Plasmid

   #81651

   Purpose

   Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   G12D

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

   Plasmid

   #141385

   Purpose

   Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-E (E SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 8, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCI-neo FlucDM EGFP

   Plasmid

   #90172

   Purpose

   EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

   Depositor

   Franz-Ulrich Hartl

   Article

   Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

   Insert

   FLuc EGFP
   

   Expression

   Mammalian

   Mutation

   R188Q, R261Q in FLuc

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11d-RAN

   Plasmid

   #108919

   Purpose

   Expresses human RAN

   Depositor

   Larry Gerace

   Article

   Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

   Insert

   RAN (RAN Human)
   

   Expression

   Bacterial

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ Grx1-roCherry

   Plasmid

   #141068

   Purpose

   Fluorescent biosensor for 2GSH/GSSG ratio

   Depositor

   Dmitry Bilan

   Article

   Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

   Insert

   Grx1-roCherry
   

   Expression

   Mammalian

   Mutation

   Changed Alanine 150 to Cysteine, inserted Threon…

   Promoter

   CMV, SP6

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. HIR WT

   Plasmid

   #24049

   Depositor

   Frederick Stanley

   Article

   Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

   Insert

   Insulin Receptor (INSR Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IPU-GFP-MYH9

   Plasmid

   #168273

   Purpose

   Expresses MYH9 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   MYH9 (MYH9 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIG3 (pCMV-IRES-GFP version 3)

   Plasmid

   #78264

   Purpose

   Lentiviral empty cloning vector containing IRES and GFP

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   CMV, lentiviral LTR

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. nAChR beta2 WT

   Plasmid

   #24272

   Depositor

   Henry Lester

   Article

   Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

   Insert

   nAChR beta2 WT (Chrnb2 Mouse)
   

   Expression

   Mammalian

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. CX3CR1-EGFP

    Plasmid

    #24061

    Depositor

    Dan Littman

    Insert

    CX3CR1 (Cx3cr1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    EGFP

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti Lifeact-mRuby2 PuroR

    Plasmid

    #84386

    Purpose

    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mRuby2

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST17-UbE2R2

    Plasmid

    #15806

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICE-HA-MRE11-WT

    Plasmid

    #82033

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. ePPE-spG

    Plasmid

    #183096

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nspGCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A in SpGCas9

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2NIL

    Plasmid

    #20188

    Depositor

    Tanya Parish

    Article

    Parish et al Microbiology (Reading). 2000 Aug;146 ( P

    Type

    Empty backbone

    Use

    Suicide vector

    Available Since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15-UbE2D1

    Plasmid

    #15782

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
    

    Tags

    6x His, T7 tag, and Xpress tag (enterokinase clea…

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. IGF1R_pLX307

    Plasmid

    #98344

    Purpose

    Lentiviral expression of IGF1R

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    IGF1R (IGF1R Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-DIO-mCherry-WPRE-pA

    Plasmid

    #37083

    Purpose

    Cre-dependent expression of mCherry

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox; Cre-on

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. EphA2-YFP

    Plasmid

    #108852

    Purpose

    Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

    Depositor

    Kalina Hristova

    Article

    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

    Insert

    EPHA2 (EPHA2 Human)
    

    Tags

    Labeled with eYFP on the C-Terminus via a 15 amin…

    Expression

    Mammalian

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.GFAP.eGFP.WPRE.hGH

    Plasmid

    #105549

    Purpose

    AAV expression of EGFP from GFAP promoter

    Depositor

    James M. Wilson

    Has Service

    AAV5

    Insert

    eGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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