We narrowed to 8,430 results for: BLI
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Plasmid#216593Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorInsertMECP2 (MECP2 Human)
UseGateway entry plasmidTagsExpressionMutationR91WPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-hHNF4aORF-1
Plasmid#206959PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.DepositorInsertHNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-56
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-191~307-mEGFP(A206K)-TwStrep
Plasmid#208626Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 191~307, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-299~398-mEGFP(A206K)-TwStrep
Plasmid#208627Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 299~398, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-212~285-mEGFP(A206K)-TwStrep
Plasmid#208629Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 212~285, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-62
Plasmid#208647PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-OH
Plasmid#138956PurposeUsed to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv with C terminal epitope tag (kkv Fly)
UseTagsollas and his-6 added to C terminus of protein co…ExpressionInsectMutationPromoterUASAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-kkv-3::NG
Plasmid#138960PurposeUsed to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.DepositorArticleInsert3' end of kkv and neon green (kkv Fly)
UseAn editing vector for drosophilaTagsNeon Green fused to C terminus of KkvExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald
Plasmid#201187PurposeLentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald
Plasmid#201188PurposeLentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067L
Plasmid#200253PurposepBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomatoDepositorInsert6 gRNAs targeting Doublesex, Intersex, bTublin
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL1P6 ZmUbiP:GRF-GIF:NosT
Plasmid#198049PurposeMoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyTagsExpressionMutationPromoterZea mays (maize) ubiquitin promoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4-Y801H Ankyrin Repeats pET151
Plasmid#164058PurposeExpresses mouse KANK4 Ank domain -Y801H mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOD_004
Plasmid#155361PurposeGentamycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with SalmonellaDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOD_003
Plasmid#155362PurposeZeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with SalmonellaDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEP4_HA-Halo-cmyc-GRM3 C127A
Plasmid#140458PurposeMammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127ADepositorInsertGRM3 (GRM3 Human)
UseTagsHalo, Signal/leader sequence from influenza hemag…ExpressionMammalianMutationCysteine 127 to alanine, Threonine 174 to alaninePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c014
Plasmid#175451PurposeProtein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.DepositorInsertCD44 (CD44 Human)
UseTagsAviTag-His6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44U-c011
Plasmid#175454PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.DepositorInsertCD44 (CD44 Human)
UseTagsAviTag-His6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c011
Plasmid#175449PurposeProtein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.DepositorInsertCD44 (CD44 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only