We narrowed to 9,391 results for: BLI

1. Lamin A (R298C)-mRFP

   Plasmid

   #124269

   Purpose

   Lamin A mutant (R298C), C-terminally tagged with Mrfp. Mutation associated with CMT neuropathy.

   Depositor

   Eric Schirmer

   Insert

   LMNA (R298C) (LMNA Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   LMNA (R298C)

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSBtet-Bla/PAG1-TurboID-GAI

   Plasmid

   #198217

   Purpose

   Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling

   Depositor

   Eric Davis

   Insert

   PAG1 (PAG1 Human)
   

   Use

   Transposon

   Tags

   3’ part of TurboID, starting with G257 and amino …

   Expression

   Mammalian

   Mutation

   None

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. huntingtin_V2095-V3138_CbioCflag

   Plasmid

   #194764

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   Avi-tag (biotin acceptor peptide) and Flag-tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV and P10

   Available Since

   July 27, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. huntingtin_T97-M2069_NbioCflag

   Plasmid

   #194763

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   Avi-tag (biotin acceptor peptide) and Flag-tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV and P10

   Available Since

   July 26, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. MAP3K3

   Plasmid

   #38937

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAP3K3 (MAPK3 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pDONR221_SMAD2_HAe2_Bsd

   Plasmid

   #167166

   Purpose

   Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.

   Depositor

   Alexander Loewer

   Insert

   Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
   

   Use

   Repair template for gene editing in human cells

   Tags

   blasticidin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SDC229-B03

   Plasmid

   #58763

   Purpose

   bacterial expression of PDB 4PYZ

   Depositor

   Cheryl Arrowsmith

   Insert

   PDB:4PYZ (USP7 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   Aug. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. Myog'-2A-mCherryNLS-PGK-Puro

   Plasmid

   #69548

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm
   Myogenin 3' homology arm (Myog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO-Tet-On-HDAC1-shRNA3

   Plasmid

   #90243

   Purpose

   Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)

   Depositor

   Roland Friedel

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Lentiviral

   Available Since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+5'MT-wbp2nl

    Plasmid

    #172467

    Purpose

    expresses myc-tagged version of wild-type protein

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nl
    

    Use

    Xenopus expression

    Tags

    Myc

    Promoter

    SP6

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ataxin3:1-431_Q79_BV

    Plasmid

    #209733

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    6xHis-SBP-TEV

    Expression

    Bacterial

    Promoter

    Polyhedrin

    Available Since

    Nov. 13, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. AKT3

    Plasmid

    #39038

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AKT3 (AKT3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. Syn-ChR-tdTomato

    Plasmid

    #99843

    Purpose

    AAV expression of ChR2(H134R)-tdTomato under the Syn promoter

    Depositor

    Gordon Shepherd

    Insert

    ChR2(H134R)-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    ChR2(H134R), carrying a single point mutation at …

    Promoter

    human synapsin I promoter

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MS2_SC2_S_Omicron

    Plasmid

    #180229

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Omicron (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. MS2_SC2_S_WT

    Plasmid

    #175044

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    WT (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. human KANK1 Ankyrin Repeats pET151

    Plasmid

    #164053

    Purpose

    Expresses human KANK1 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

    Plasmid

    #39300

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S16A, R17A, H18A, D19A, W23A and N25A

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. CYP7A1 (3SN5)

    Plasmid

    #32874

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP7A1 (CYP7A1 Human)
    

    Expression

    Bacterial

    Mutation

    amino acids 25-503 only

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. AAV-SYN-PIK3CD

    Plasmid

    #203730

    Purpose

    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASp YFP Rac1 CA

    Plasmid

    #52235

    Purpose

    Drosophila expression of YFP-Rac1 constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Rac1 (Rac1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only