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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-BS2-TRFLE

Plasmid

#247993

Purpose

BS2-TRFLE bacterial expression plasmid

Depositor

Jeffrey Martell

Article

Ogorek et al ACS Cent. Sci. 2026,

Insert

BS2-TRFLE Esterase

Tags

His Tag

Expression

Bacterial

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pEF6a-CD28-PafA

Plasmid

#113400

Purpose

Expresses CD28-PafA in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

CD28-PafA

Tags

myc

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-CAG -IRES-GFP

Plasmid

#69047

Purpose

Lentiviral backbone for GFP coexpression

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II 1-22

Plasmid

#35178

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of C-terminal domain repeats 23-52

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Pol-II 31-52

Plasmid

#35179

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of C-terminal domain repeats 1-30

Promoter

CMV

Available Since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only
pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

Plasmid

#171104

Purpose

Expresses sgRNA (ENO1 gRNA) in Candida auris.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

sgRNA

Expression

Yeast

Promoter

ADH1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSelAct-KO

Plasmid

#174374

Purpose

Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible

Depositor

Gitta Coaker

Article

Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

Type

Empty backbone

Use

Vector for 2-step knockout by homologous recombin…

Promoter

N/A

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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HA-NFAT4(3-407)-GFP

Plasmid

#21664

Depositor

Anjana Rao

Article

Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

Insert

NFAT4 (NFATC3 Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

Amino acids 3-407

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pKL09-GCaMP6f-mScarlet bb118

Plasmid

#158979

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-mScarlet fusion

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0679

Plasmid

#149689

Purpose

Bacterial expression of SENP_EuH protease

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SENP_EuH protease

Tags

His14-tag and Tev protease cleavage site

Expression

Bacterial

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-T1ER

Plasmid

#47928

Purpose

T1ER is a calcium sensor targeted to ER

Depositor

Tobias Meyer

Article

Bandara et al Sci Signal. 2013 Jul 9;6(283):ra56. doi:

Insert

T1ER

Expression

Mammalian

Mutation

KDEL

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pNIR-FbALFA

Plasmid

#184678

Purpose

NIR-Fb to ALFA-tag

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbALFA

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pPlantPOP_STARBURST

Plasmid

#241284

Purpose

UBQ10 driven expression of STARBURST, a poly-cistronic fungal bioluminescense pathway, in plants

Depositor

Sean Cutler

Article

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Insert

STARBURST

Expression

Plant

Mutation

NA

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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PB-HSPB-Cre

Plasmid

#235217

Purpose

Cre expression under the control of HSPB promoter; Tet-On 3G-inducible PiggyBac system for expression in human pluripotent stem cells.

Depositor

Xiaojun Lian

Article

Howells et al Biotechnol Bioeng. 2025 Aug 20. doi: 10.

Insert

HSPB-Cre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-FAF1

Plasmid

#107986

Purpose

expression of FAF1

Depositor

Georg Weber

Article

Weber et al Sarcoma. 2015;2015:839182. doi: 10.1155/

Insert

FAF1 (FAF1 Human)

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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LZRS-IresGFP

Plasmid

#21961

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

Ires GFP

Use

Retroviral

Expression

Mammalian

Mutation

GACAGATGACTATGCAGAGAT

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pCEP9 MLH1

Plasmid

#16458

Depositor

Bert Vogelstein

Article

Papadopoulos et al Science. 1994 Mar 18. 263(5153):1625-9.

Insert

MLH1 (MLH1 Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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1B-GFP1-10

Plasmid

#193867

Purpose

E. coli expression vector for Hisx6 tagged GFP1-10.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

sfGFP1-10

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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