We narrowed to 16,181 results for: nes
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Plasmid#206026PurposeAn expression vector for mouse LINE1 ORF2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertORF2 derived from mouse LINE1
ExpressionMammalianAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP13A3 D498N
Plasmid#195850PurposeExpresses D498N mutant Homo sapiens ATP13A3DepositorAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_HT_B_6xHis-TEV-GFP-NBR1
Plasmid#190928PurposePlasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914DepositorAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mTagBFP-T2A-TetOn3G
Plasmid#179888PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsmTagBFPExpressionBacterial and MammalianPromoterEF1aAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPyCAG-NE-mKate2-IRES-Hph
Plasmid#183616PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.DepositorInsertmKate2
TagsHuman EMD transmembrane domain and NLSExpressionMammalianPromoterCAGAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode
Plasmid#170220PurposeBarcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU315
Plasmid#169697PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags1xHA tagExpressionBacterialAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUB7
Plasmid#169698PurposeTemplate plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags6xHis tagExpressionBacterialAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170809PurposeLentiviral vector to co-express an spsgRNA with NLS-mNeonGreenDepositorTypeEmpty backboneUseLentiviralExpressionMammalianMutationNAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170810PurposeLentiviral vector to co-express an spsgRNA with NLS-mScarlet-IDepositorTypeEmpty backboneUseLentiviralExpressionMammalianMutationNAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
Tags6xHis tag and DsbA signal sequence for periplasmi…ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-GPT2-3xFLAG
Plasmid#163449Purposelentiviral overexpression vector for human GPT2DepositorInsertGPT2 (GPT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mKate-MKLP1
Plasmid#140570PurposeExpresses mKate tagged MKLP1DepositorAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk12_Untagged
Plasmid#127176PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UseEntry vector with transgene for gateway cloningTagsNonePromoterNoneAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
UBC64
Plasmid#25143PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertubc64.248.414 (UBE2Q1 Human)
TagsHis tag with thrombin cleavageExpressionBacterialMutationcontains UBC domain; residues 248-414Available sinceOct. 31, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEn-C1.1
Plasmid#61479PurposeEncodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPCDepositorInsertAtU6-26:sgRNA
UseCRISPRExpressionPlantPromoterU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-PYK1
Plasmid#85781PurposeN-ICE plasmid pH3FS-PYK1DepositorInsertloxP-HphMX-loxP-PYK1p-3FLAG
UseCre/LoxExpressionYeastAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
tubulin-ReNL_pcDNA3
Plasmid#89530PurposeRed color variant of bright luminescent protein, fused to b-tubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertReNL
Tagsb-tubulinExpressionMammalianPromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cox8x2-YeNL_pcDNA3
Plasmid#89517PurposeYellow color variant of bright luminescent protein, targeted to the MitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYeNL
TagsCoxVIII X2ExpressionMammalianPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cox8x2-ReNL_pcDNA3
Plasmid#89533PurposeRed color variant of bright luminescent protein, targeted to the MitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertReNL
TagsCoxVIII X2ExpressionMammalianPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only