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MTK3_002

Plasmid

#123719

Purpose

Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Blasticydin Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pICH54011

Plasmid

#48065

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pICH54022

Plasmid

#48066

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pNC860

Plasmid

#20390

Depositor

Beverly Errede

Article

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP

Expression

Yeast

Mutation

Linker: ek

Available Since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pSEVA621-AzFRS

Plasmid

#242002

Purpose

Expressed AzF tRNA synthetase for incorporation of p-azido-L-phenylalanine (pAzF)

Depositor

Wei Niu

Article

He et al ACS Synth Biol. 2022 Nov 18;11(11):3724-

Insert

AzFRS

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-α1

Plasmid

#242515

Purpose

Donor II vector in DASH and alpha1 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-ω2

Plasmid

#242518

Purpose

Donor II vector in DASH and omega2 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pQE81XN-mGold

Plasmid

#218959

Purpose

Expresses Hisx6-Tagged mGold in bacteria

Depositor

Benjamin Glick

Insert

mGold

Tags

6X-His

Expression

Bacterial

Promoter

T5

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Promoter

tight TRE

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTG612

Plasmid

#237387

Purpose

mStayGold^NSEC^ repair template

Depositor

Ariel Pani

Article

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

mStayGold^NSEC^

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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proinsulin-EYFP

Plasmid

#218952

Purpose

Expresses proinsulin fused to EYFP in bacteria

Depositor

Benjamin Glick

Insert

proinsulin-EYFP

Tags

proinsulin

Expression

Bacterial

Promoter

T5

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8317

Plasmid

#213989

Purpose

PrpsM driven expression of sfGFP

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

sfGFP

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8731

Plasmid

#213991

Purpose

PrpsM driven expression of mNeonGreen - sequence codon-optimized for Salmonella

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM10686

Plasmid

#213995

Purpose

PrpsM driven expression of mScarlet-I

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8313

Plasmid

#213986

Purpose

PrpsM driven expression of mCerulean

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKERep

Plasmid

#211826

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMP118

Plasmid

#207947

Purpose

GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mVenus

Plasmid

#154898

Purpose

mVenus entry vector

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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OA-16

Plasmid

#104966

Purpose

Plasmid expressing two multiplexed ribozyme-gRNA-ribozyme (RGR) gRNAs

Depositor

Omar Akbari

Article

Marshall et al Sci Rep. 2017 Jun 19;7(1):3776. doi: 10.

Insert

Ribozyme-gRNA-ribozyme

Use

Cre/Lox

Expression

Insect

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM1263

Plasmid

#47985

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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