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pMS6: pHelper(AvCAST)_entry_ΔTnsD

Plasmid

#168139

Purpose

Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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DNMT1 KO Hyg

Plasmid

#16648

Depositor

Bert Vogelstein

Article

Rhee et al Nature. 2002 Apr 4. 416(6880):552-6.

Insert

DNMT1 knock-out construct (DNMT1 Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pLenti-X1-Neo-HA-UFL1-RAMP4

Plasmid

#139863

Purpose

Lentiviral expression of fusion of human UFL1 (full length) with RAMP4

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFL1-RAMP4 (UFL1 Human)

Use

Lentiviral

Tags

HA

Mutation

recombinant fusion of UFL1 (full length) with RAM…

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMS22: pHelper(PmcCAST)_entry_ΔTniQ

Plasmid

#168155

Purpose

Inducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ

Plasmid

#168152

Purpose

Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS5: pHelper(AvCAST)_entry_ΔTniQ

Plasmid

#168138

Purpose

Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS4: pHelper(AvCAST)_entry

Plasmid

#168137

Purpose

Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ

Plasmid

#168134

Purpose

Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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KHBD00607

Plasmid

#39685

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG32788 (Crg-1 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3/Myc-DNMT1

Plasmid

#36939

Purpose

Mammalian expression of human DNMT1 with myc tag

Depositor

Arthur Riggs

Article

Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

Insert

DNMT1 (DNMT1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-hCas3

Plasmid

#134920

Purpose

Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas3 with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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7TFP CDH1 reporter

Plasmid

#91704

Purpose

lentiviral luciferase reporter containing CDH1 promoter

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

CDH1 promoter (CDH1 Human)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

CDH1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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FR_HNF1B

Plasmid

#31101

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

wild type

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pSMP-DNMT1_1

Plasmid

#36382

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

DNMT1 (DNMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-DNMT1_2

Plasmid

#36383

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

DNMT1 (DNMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
FAK2_HUMAN_D0

Plasmid

#79747

Purpose

This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FAK2 (PTK2B Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
HTRA1_PDZ_1

Plasmid

#103912

Purpose

Protein expression and purification of HTRA1 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

HTRA1 (HTRA1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-NLS-Beta-catenin-S33Y

Plasmid

#138537

Purpose

For making Beta-catenin-S33Y RNA

Depositor

Carmen Williams

Article

Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

Insert

Beta-catenin

Use

In vitro transcription

Tags

2XFLAG and 2xNLS

Mutation

changed serine 33 to tyrosine

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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DNMT1 KO Neo

Plasmid

#16649

Depositor

Bert Vogelstein

Article

Rhee et al Nature. 2002 Apr 4. 416(6880):552-6.

Insert

DNMT1 knock-out construct (DNMT1 Human)

Expression

Bacterial

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
FR_HNF1B_A263insGG

Plasmid

#31103

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

mutation A263insGG

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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