We narrowed to 17,786 results for: Met;

1. pAAVsc CB6PI Gpluc7XmiR-122T

   Plasmid

   #35647

   Depositor

   Phillip Zamore

   Article

   Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

   Insert

   7 bulged miR-122 target sites
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CB

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. ST-SBP-EGFP_puromycin

   Plasmid

   #65270

   Purpose

   ST reporter only ( no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO1-puro-U6-sgRNA-TGM2

   Plasmid

   #69239

   Purpose

   U6 driven SpCas9 sgRNA expression for TGM2 site

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TGM2 sgRNA (TGM2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-P1-N-EGFP-C

   Plasmid

   #159436

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-P1-N-miRFP670-C

   Plasmid

   #159438

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Insert

   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. spa-GCaMP6s

   Plasmid

   #67556

   Purpose

   photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6s
   

   Use

   Tags

   HIS-tag

   Expression

   Mammalian

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pENTR1A-3xFlag-M694V-MEFV

   Plasmid

   #167118

   Purpose

   Gateway entry plasmid with 3xFlag-pyrin (M694V/FMF variant)

   Depositor

   Thomas Henry

   Article

   Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

   Insert

   MEFV (MEFV Human)
   

   Use

   Gateway entry vector

   Tags

   3xFlag

   Expression

   Mutation

   M694V Methionine 694 to valine (FMF mutation)

   Promoter

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

   Plasmid

   #89872

   Purpose

   expresses CB1R-iTango component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   CB1R-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU1/Sm/3' Box_(GLuc)_INT

   Plasmid

   #68438

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U1

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Str-KDEL_ManII-SBP-tagBFP

    Plasmid

    #65254

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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