We narrowed to 9,755 results for: SUB

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Expression

Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-p47PX(aa2-132)

Plasmid

#19007

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p47phox (NCF1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa2-132 (PX domains)

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pRSF-1b-S2

Plasmid

#128590

Purpose

Expresses tagless S2 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S2 (rpsB E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLG015 (RADAR)

Plasmid

#157893

Purpose

Expresses the RADAR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

RADAR subtype containing auxillary gene rdrD

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pACλCI-β-flap (831-1057)

Plasmid

#53733

Purpose

Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

λCI-β-flap fusion

Expression

Bacterial

Promoter

lac UV5

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-Sec61β

Plasmid

#172442

Purpose

Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Tags

FKBP-GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP(C1)-PP1gamma

Plasmid

#44225

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP-1G (PPP1CC Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pL-rpb1-N488D

Plasmid

#10988

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-N488D (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 1462 A to G in the RPB1 ORF resulting in…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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Cherry-ICP0-C3

Plasmid

#134560

Purpose

Expresses Cherry-ICP0 fusion protein

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLVET-tTR-KRAB

Plasmid

#11644

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hEF-1alpha, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-zif268

Plasmid

#18045

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

zif268

Expression

Bacterial

Mutation

Zif268 is fused to the omega subunit of RNA polym…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pLVCT-rtTR-KRAB-2SM2

Plasmid

#11779

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCZGY3163

Plasmid

#101248

Purpose

GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

gfp11::rps-18 (rps-18 Synthetic, Nematode)

Use

Gateway

Tags

GFP11

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-FnCpf1

Plasmid

#129554

Purpose

Contains codon-optimized FnCpf1 and gRNA for streptomyces

Depositor

Fong Tian Wong, Mingzi M. Zhang

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

FnCpf1

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/Rabaptin-5:R5BD

Plasmid

#168808

Purpose

Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

Depositor

Guangpu Li

Article

Qi et al Methods Mol Biol. 2015;1298:259-70. doi:

Insert

Rab5-binding domain (aa739-862) of Rabaptin-5

Tags

GST

Expression

Bacterial

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pJB06

Plasmid

#190480

Purpose

Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Cas9
XylR

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Promoter

xylR-driven promoter

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJB07

Plasmid

#190481

Purpose

Targeting plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Upstream & Downstream pyrE deletion region
gRNA

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB-2SM2

Plasmid

#11652

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB-2SM2, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pAV0279

Plasmid

#149688

Purpose

Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SUMO_Eu1-MBP

Tags

3xFLAG-tag and His14-tag

Expression

Bacterial

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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