We narrowed to 12,327 results for: pcDNA

Showing: 7141 - 7160 of 12327 results

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pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

Plasmid

#226516

Purpose

Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

TOMM20-SNAP-tag2-T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

Plasmid

#175524

Purpose

Co-expression of HaloTag7 (golgi) and SNAPf-tag (membrane inner leaflet) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag7 and Lyn11-SNAPf

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKlab1194-pcDNA-3.1-BACH1/BRIP1/FANCJ-3xFLAG-V5

Plasmid

#249002

Purpose

This plasmid expresses the BACH1 protein sequence for pull down assays

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BACH1/BRIP1/FANCJ (BACH1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/myc-His-A-YAP2-1st&2nd WW mut

Plasmid

#19061

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine,and Proline 202…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

Plasmid

#202606

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-P2A-mCherry-CAAX

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

Plasmid

#79620

Purpose

Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1 and UGI

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

Plasmid

#124494

Purpose

Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Caspase-1 cleavage seq) T2A mCherry

Use

Synthetic Biology

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

Plasmid

#175525

Purpose

Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag10 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-3XFLAG-miniTurbo-NLS-VHL54:213

Plasmid

#248671

Purpose

To generate inducible Flp-In cells expressing Nterm tagged miniTurbo deltaVHL for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL(54-213) (VHL Human)

Tags

FLAG-miniTurbo-NLS

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

Plasmid

#175523

Purpose

Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag11 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

Plasmid

#175547

Purpose

Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

Plasmid

#226515

Purpose

Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

Cox8a(4x)-SNAP-tag2-T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

Plasmid

#188994

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS

Plasmid

#194751

Purpose

To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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Myc-tagged human full length TCF4E pcDNA3

Plasmid

#32738

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

TCF4E (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

Plasmid

#225588

Purpose

Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Article

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

T21R-F8.2(S54L+T127A)-T2A-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-3xFLAG-His6-LARP1-d327-496

Plasmid

#233627

Purpose

Expression of Flag- and His-tagged LARP1delta327-496

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

LARP1delta327-496 (LARP1 Human)

Tags

3FLAG-His6

Expression

Mammalian

Mutation

delta327-496

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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(344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

Plasmid

#168097

Purpose

HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

HA-nGFP-(EAAAK)4-OGA(544-706)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

Plasmid

#124493

Purpose

Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipYFP(TEV cleavage seq) T2A mCherry

Use

Synthetic Biology

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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