We narrowed to 15,838 results for: grna

1. CAG-mCherry-RORbshRNA1

   Plasmid

   #73983

   Purpose

   RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse RORb gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAWK

   Plasmid

   #62713

   Purpose

   Donor vector for amiR-eGFP knock-in via landing pad

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   amiR-eGFP123/amiR-eGFP419/FLPe
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pARA (pKD-G1)

   Plasmid

   #62680

   Purpose

   Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   tdTomato/amiR-eGFP123
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pARB (pKD-G2)

   Plasmid

   #62681

   Purpose

   Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   tdTomato/amiR-eGFP419
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pARD (pKD-G4)

   Plasmid

   #62683

   Purpose

   Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   amiR-eGFP419
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pATJ (pKD-G8)

   Plasmid

   #62687

   Purpose

   Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   amiR-eGFP419/amiR-eGFP123
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBLV B4

   Plasmid

   #68057

   Purpose

   expresses BLV (NC001414) B4 pre-miRNA and miRNAs

   Depositor

   Christopher Sullivan

   Article

   Burke et al Nucleic Acids Res. 2014 Dec 16;42(22):13

   Insert

   BLV-miR-B4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pX330A_FokI-1x5

   Plasmid

   #63605

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAN-OR-MalT-3NT

   Plasmid

   #62309

   Purpose

   synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Inserts

   A2NT sgRNA
   Malt-3NT sgRNA
   A2NT sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PA2, PPhlF, and pBAD

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSicoR-RPL30i1

    Plasmid

    #59626

    Purpose

    Expression of shRNA against human RPL30

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    RPL30 (RPL30 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSicoR-Ti2

    Plasmid

    #59631

    Purpose

    Expression of shRNA against human T

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    T
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pSicoR-Ti1

    Plasmid

    #59630

    Purpose

    Expression of shRNA against human T

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    T
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pSicoR-RPL30i2

    Plasmid

    #59627

    Purpose

    Expression of shRNA against human RPL30

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    RPL30 (RPL30 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSicoR-ARSDi2

    Plasmid

    #59601

    Purpose

    Expression of shRNA against human ARSD

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    ARSD (ARSD Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pSicoR-UBE2E3i2

    Plasmid

    #59592

    Purpose

    Expression of shRNA against human UBE2E3

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    UBE2E3 (UBE2E3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pSicoR-UBE2E3i1

    Plasmid

    #59590

    Purpose

    Expression of shRNA against human UBE2E3

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    UBE2E3 (UBE2E3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pLL3.7 Alk.2 shRNA

    Plasmid

    #59297

    Purpose

    shRNA to mouse Alk

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

    Insert

    Alk.2 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLL3.7 Lmo3.5 shRNA

    Plasmid

    #59295

    Purpose

    shRNA to mouse Lmo3

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al Alcohol Clin Exp Res. 2011 Sep;35(9):160

    Insert

    Lmo3.5 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pLL3.7 Lmo3.8 shRNA

    Plasmid

    #59294

    Purpose

    shRNA to mouse Lmo3

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al Alcohol Clin Exp Res. 2011 Sep;35(9):160

    Insert

    Lmo3.8 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pSUPER.retro.puro-THAP11-A

    Plasmid

    #36993

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 shRNA (THAP11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pSUPER.retro.puro-THAP11-C

    Plasmid

    #36994

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 shRNA (THAP11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pSUPER.retro.puro-THAP11-E

    Plasmid

    #36995

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 shRNA (THAP11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

23. MDH1-pre-let-7_PShairpin

    Plasmid

    #26537

    Depositor

    Chang-Zheng Chen

    Article

    Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

    Insert

    pre-let-7_PS hairpin
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    5 nt mutated to make pre-let-7 stem pairing perfe…

    Promoter

    Available Since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO.1-blast-cfRab8b_1

    Plasmid

    #26708

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    dog Rab8b RNAi
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Dog Rab8b RNAi

    Promoter

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

25. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid

    #118162

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid

    #118163

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid

    #118164

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid

    #118161

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lentiCRISPR v2

    Plasmid

    #52961

    Purpose

    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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