We narrowed to 1,429 results for: tk
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Plasmid#47616Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron fragment (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZExpressionMutation714 bp from 3' end of 2nd intron with a dele…PromoterHSV tk promoterAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
Myc-RILPL1
Plasmid#201470PurposeFor overexpression in cultured cells to visualize RILPL1 protein interactions.DepositorAvailable SinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAG491
Plasmid#226747PurposeCRISPRi guide RNA against MTCH2 (dual guide)DepositorAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM234
Plasmid#220177PurposeExpresses exogenous wild-type NAC complex members (NACA and BTF3)DepositorUseLentiviralTags3xFLAG (NACA); 6xHis (BTF3)ExpressionMutationPromoterAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA
Plasmid#55202PurposePlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dCas9-3xNLS-VP64 (Construct 1)
Plasmid#55195PurposeExpresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsVP64ExpressionMammalianMutationPromoterCMV/hUBCAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Human, Synthetic)
UseTagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-Amp
Plasmid#108707PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM643
Plasmid#112533PurposepZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
UseTagsExpressionBacterialMutationA17PPromoterJ23107, PL(HrtO), and ProDAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Flag-H2A K13-15-118-119R
Plasmid#63568PurposeExpression of human H2A (with multiple Lysine to Arginine point mutations) tagged with FlagDepositorInsertH2A (H2AC20 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationLysine at 13, 15, 118, and 119 are now ArgininePromoterCMVAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R
Plasmid#63565PurposeExpression of human H2A (with multiple Lysine to Arginine point mutations) tagged with FlagDepositorInsertH2A (H2AC20 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationLysines at 5, 9, 118, 119, 125, 127, and 129 are…PromoterCMVAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD
Plasmid#159999PurposeExpresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorInsertSpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastbac1-GST-hUSP7
Plasmid#63573PurposeExpression of synthetic human USP7 tagged with GSTDepositorInsertUSP7 (USP7 Human, Synthetic)
UseTagsGSTExpressionInsectMutationSynthetic based on human protein sequence (please…PromoterPolyhedrinAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG27
Plasmid#226742PurposeExpresses a reporter for a mitochondrial matrix proteinDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM79
Plasmid#220171PurposeCRISRPi guide RNA against MARCHF5DepositorAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLD-CAG-DYSF-CFL
Plasmid#131457PurposeEncodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imagingDepositorUseTagsExpressionBacterial and MammalianMutationR15C (in DYSF) and Q2579H (in CFL)PromoterAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEXNKI-his-GST-3C-LIC
Plasmid#108709PurposeExpression of a GST-6xHis-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsGST-6xHis-3C protease cleavage siteExpressionBacterialMutationPromotertacAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG eGFP-Oct4
Plasmid#203789PurposeCompetitive assay of Oct4 and Oct6 binding to DNA motifs.DepositorAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-kan
Plasmid#108703PurposeExpression of an His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-his-3C-LIC-strep
Plasmid#108705PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))
Plasmid#105210PurposeModule 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)DepositorInsertSSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-kan
Plasmid#108706PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))
Plasmid#105207PurposeModule 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)DepositorInsertSSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)
Plasmid#105192PurposeModule 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)DepositorInsertS(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))
Plasmid#105209PurposeModule 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)DepositorInsertSSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-Amp
Plasmid#108704PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)
Plasmid#105202PurposeModule 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)DepositorInsertS(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG392
Plasmid#226749PurposeCRISPRi guide RNA against ATP13A1DepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only