We narrowed to 2,540 results for: organization Addgene
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Plasmid#127357Purposegateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPMutationR118GPromoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-CHAD
Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SIK1
Plasmid#51577PurposeConstitutively express nucleolus localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsSIK1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SNF7
Plasmid#51574PurposeConstitutively express endosome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsSNF7 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZJOM108
Plasmid#133665PurposeIntegration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorAvailable SinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLV-EF1a-V5-Vrk2
Plasmid#120240PurposeExpresses V5-tagged Vrk2 in lentiviral vectorDepositorAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG_withTEVsite
Plasmid#124940PurposeExpression of RGG-RGGDepositorInsertRGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHisExpressionBacterialMutationwith TEV site between RGG domainsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RFP-RGG
Plasmid#124944PurposeExoression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHis and mCherryExpressionBacterialAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM12
Plasmid#133638PurposeIntegration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFE-vcDAB
Plasmid#133004PurposepFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)DepositorInsertsvcdabB
vcdabA
ExpressionBacterialPromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSU4mg10
Plasmid#126079PurposePlant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cellsDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantPromoter'long' CaMV35SAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV Aquamarine-shadowY tandem
Plasmid#157775PurposeProduces a fusion between Aquamarine and shadowY. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneTagsAquamarine and shadowYExpressionMammalianAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_I392N-talin1 (1-2541)
Plasmid#166114Purposeexpresses talin-1-I392N in mammalian cellsDepositorAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rat SERCA3 (pMT2)
Plasmid#75185PurposeMammalian expression of ATP2A3DepositorAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N1 cyclin B1 L45A (1324)
Plasmid#61851Purposemammalian expression of cyclin B1 L45 A mutant fused to VenusDepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZJOM8
Plasmid#133671PurposeIntegration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-3
Plasmid#126054PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-1
Plasmid#126056PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP (F64L, S65T, V163A)-His3MX6
Plasmid#53185Purposechromosomal tagging with stable GFPDepositorAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
TPC2-mCherry
Plasmid#240468PurposeRed-tagged lysosomal channelDepositorInsertTPC2N2 (TPCN2 Human)
TagsmCherryExpressionMammalianMutationMutated aspartate 276 to lysine.PromoterCMVAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTwist-iS-methadoneSnFR-Golgi
Plasmid#220865PurposeMammalian cell expression for subcellular pharmacokinetics with Golgi targeting of iS-methadoneSnFRDepositorInsertiS-methadoneSnFR
ExpressionMammalianAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHHM.X513.iS-methadoneSnFR
Plasmid#220861PurposeBacterial expression of iS-methadoneSnFR for purification and in vitro assays.DepositorInsertiS-methadoneSnFR
TagsHis6-HA and MycExpressionBacterialAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mCherry)
Plasmid#233542PurposeA CMV promoter driving expression of a murine dominant negative myc tagged TCF4.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)
Plasmid#232128PurposeA middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)
Plasmid#232124PurposeA middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)
Plasmid#232123PurposeA middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)
Plasmid#232131PurposeA middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)
Plasmid#232130PurposeA middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1036 (pME_V5_TCF7L2_P2A_H2A_mCherry)
Plasmid#232126PurposeA middle entry gateway clone containing V5 tagged human TCF7L2 / TCF4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(711-800)_L786A
Plasmid#228838PurposeInsect cell expression of the mutant C-terminus of BICD2DepositorAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(711-800)
Plasmid#228833PurposeInsect cell expression of CC3 domain of BICD2 (aa 711-800)DepositorAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(342-800)
Plasmid#228832PurposeInsect cell expression of C-terminal portion of BICD2 (aa 342-800)DepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(711-800)_L742A
Plasmid#228839PurposeInsect cell expression of the mutant C-terminus of BICD2DepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-GFP-Rab5
Plasmid#228139PurposeExpresses GFP-Rab5 in mammalian cellsDepositorAvailable SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-SNAP-Omp25
Plasmid#228137PurposeExpresses SNAP-Omp25 in mammalian cellsDepositorInsertOmp25 (Synj2bp Rat)
UseRetroviralTagsSNAPExpressionMammalianMutationtransmembrane region (C-terminus)Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121A wgMDH
Plasmid#204252PurposeBacterial expression of G121A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG121AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196E wgMDH
Plasmid#204272PurposeBacterial expression of R196E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR196EAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123A wgMDH
Plasmid#204254PurposeBacterial expression of P123A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP123AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130Q wgMDH
Plasmid#204268PurposeBacterial expression of R130Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR130QAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127F wgMDH
Plasmid#204264PurposeBacterial expression of G127F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG127FAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126R wgMDH
Plasmid#204261PurposeBacterial expression of P126R wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP126RAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127A wgMDH
Plasmid#204263PurposeBacterial expression of G127A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG127AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 M66L wgMDH
Plasmid#204247PurposeBacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationM66LAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127P wgMDH
Plasmid#204265PurposeBacterial expression of G127P wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG127PAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121F wgMDH
Plasmid#204253PurposeBacterial expression of G121F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationG121FAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 M91Q wgMDH
Plasmid#204250PurposeBacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationM91QAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D87L wgMDH
Plasmid#204248PurposeBacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationD87LAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126G wgMDH
Plasmid#204260PurposeBacterial expression of P126G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationP126GAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only