We narrowed to 883 results for: Anti-CRISPR

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scFv-GCN4-sfGFP-TET1CD

Plasmid

#184439

Purpose

TET1 catalytic domain fused with sfGFP and scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

scFv-GCN4-sfGFP-TET1CD

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFP-VP64-GB1

Plasmid

#141417

Purpose

Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

Depositor

Izuho Hatada

Article

Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

Insert

scFvGCN4sfGFP-VP64-GB1

Tags

HA and sfGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD

Plasmid

#235574

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD

Plasmid

#235579

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD-CatMut

Plasmid

#235583

Purpose

Dox-inducible expression of control catalytic-mutant Ring1b CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Mutation

I53S

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD-CatMut

Plasmid

#235588

Purpose

Dox-inducible expression of control catalytic-mutant Setd2 CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Mutation

R1599C

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

Plasmid

#215769

Purpose

encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

Depositor

Dimitrios Wagner

Article

Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

Inserts

TRAC-5' homology arm (TRAC Human)
P2A-CD19.28.zeta-bgH (CD19 Mouse, Bovine, Human)
TRAC-3' homology arm (TRAC Human)

Expression

Bacterial

Mutation

Cas12a PAM mutated

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

Plasmid

#79373

Purpose

Recruits VPR to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-VPR

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA_887

Plasmid

#201164

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_886

Plasmid

#201162

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_888

Plasmid

#201165

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

Plasmid

#79372

Purpose

Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-p65-hsf1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-scFv

Plasmid

#78900

Purpose

Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

scFV-VP64

Use

CRISPR

Tags

GFP

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051d_sgCiPELO #1

Plasmid

#229017

Purpose

Expression of CRISPRi PELO sgRNA #1

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172436

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

CRISPR and Lentiviral

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051d_sgCiPELO #2

Plasmid

#229018

Purpose

Expression of CRISPRi PELO sgRNA #2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-ACSL3-sfGFP(C) HDR template

Plasmid

#129413

Purpose

HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

ACSL3 HDR template (ACSL3 Human)

Use

CRISPR and TALEN; Endogenous tagging hdr template

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTE_23 - pDonor_TRAC-2a-FMC6.3-BBz-2a

Plasmid

#252019

Purpose

Donor for CD19 CAR knock-in at TRAC locus

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

2a-FMC6.3-BBz-2a

Use

Unspecified

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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