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Addgene
Showing: 701 - 720 of 1414 results
  1. pOPTXcGMPRELUC

    Plasmid
    #68503
    Purpose
    cyclic nucleotide reporter for bacteria or plant cells
    Depositor
    Insert
    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    Use
    Tags
    luciferase
    Expression
    Bacterial and Plant
    Mutation
    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…
    Promoter
    OPTX promoter with cGMPRE
    Available since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pLSU1/MtHp:nnLuz-I{PIV-216}:NosT

    Plasmid
    #212192
    Purpose
    This binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the strong Medicago trunculata MtHP promoter and UTR
    Depositor
    Insert
    nnLuz-I{PIV-216}
    Use
    Tags
    Expression
    Plant
    Mutation
    PIV intron added at bp 216, eliminates expression…
    Promoter
    Medicago trunculata MtHP promoter
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. PB-GFP-Gal8

    Plasmid
    #127191
    Purpose
    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.
    Depositor
    Insert
    GFP-Gal8 (LGALS8 Human)
    Use
    Tags
    Gal8 is fused to the c-terminus of GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid
    #216732
    Purpose
    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes
    Depositor
    Insert
    sgCTG lentiviral guide RNA with eGFP tag
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

    Plasmid
    #197952
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.
    Depositor
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26 and pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pC1300_pUB10_pcoCASphi_E9t_V2_2x35Sp_HSP18t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197959
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by 2x35Sp and flanked by ribozymes.
    Depositor
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S promoter and pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pC1300_pUB10_pcoCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197960
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by UBQ10 gene promoter and flanked by ribozymes.
    Depositor
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. CMV:AsCpf1-2A-GFP-U6-GFP-sg

    Plasmid
    #194717
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP
    Depositor
    Insert
    AsCpf1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 701 - 720 of 1414 results