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pCMV HA hRb delta CDK

Plasmid

#58906

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-CDK2

Plasmid

#172613

Purpose

Expresses HA-tagged CDK2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK2 (CDK2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-ISG15 C78SN13Y

Plasmid

#165106

Purpose

bacterial expressio of a GST fusion of Human ISG15 C78SN13Y

Depositor

Arthur Haas

Article

Narasimhan et al J Biol Chem. 2005 Jul 22. 280(29):27356-

Insert

ISG15 (ISG15 Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

C78SN13Y; The sequence of ISG15-C78S/N13Y contai…

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-CDK4

Plasmid

#172612

Purpose

Expresses HA-tagged CDK4 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK4 (CDK4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-CCND1

Plasmid

#172649

Purpose

Expresses HA-tagged cyclin D1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-45b-HP1a-mCherry

Plasmid

#185012

Purpose

Bacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

CBX5 (HP1a) (Cbx5 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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p3E ubb polyA

Plasmid

#195977

Purpose

Gateway p3E vector

Depositor

Christian Mosimann, Alexa Burger

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubb:polyA (ubb Zebrafish)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1424

Plasmid

#29220

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple155-EGFP-NLS

Use

High copy number homologous recombination plasmid…

Tags

Expression

Mammalian

Mutation

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CCND1

Plasmid

#172632

Purpose

Expresses untagged cyclin D1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1062

Plasmid

#29011

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple155-Cre/EGFP-NLS

Use

High copy number homologous recombination plasmid…

Tags

Expression

Mammalian

Mutation

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1626

Plasmid

#29281

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple155-Intron-LacZ-NLS

Use

High copy number homologous recombination plasmid…

Tags

Expression

Mammalian

Mutation

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1097

Plasmid

#29028

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple201 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1430

Plasmid

#29225

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple170 (POGZ Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1641

Plasmid

#29283

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple170 (POGZ Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Nov. 3, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1673

Plasmid

#29290

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple201 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMS1707

Plasmid

#29295

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple235 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2374

Plasmid

#225631

Purpose

High Copy number plasmid containing Ple391 (DRD2 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple391 (DRD2 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2368

Plasmid

#225632

Purpose

High Copy number plasmid containing Ple385 (GPR6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple385 (GPR6 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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(attB) tdMCP-tagged BRaf

Plasmid

#226165

Purpose

Used to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

3xFlag-tdMCP-BRaf (BRAF Human)

Use

Bxb1 attb, no promoter

Tags

3xFlag-tdMCP and IRES-mCherry-P2A-PuroR

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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