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We narrowed to 1,028 results for: biotin

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Showing: 701 - 720 of 1028 results

  1. PTRAMP-bio

    Plasmid
    #47793
    Purpose
    Expresses enzymatically monobiotinylated full-length PTRAMP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised PTRAMP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47793-plasmid-map-sequence-id-68770

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  5. TRIPZ-bioGEF-SUMO1nc-PURO

    Plasmid
    #208042
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208042-plasmid-map-sequence-id-410263

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  6. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid
    #208043
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO2nc (SUMO2 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    Q90P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208043-plasmid-map-sequence-id-410267

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  7. LRP1A-c004

    Plasmid
    #205112
    Purpose
    LRP1 protein expression
    Depositor
    Paul Brennan
    Insert
    LRP1 (LRP1 Human)
    Tags
    AviTag and GP64sp-6His-TEV
    Expression
    Insect
    Mutation
    WT
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205112-plasmid-map-sequence-id-412366

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  8. pDS170-enNTS1

    Plasmid
    #199152
    Purpose
    JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)
    Depositor
    Daniel Scott
    Publication
    Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:
    Insert
    enNTS1 (Ntsr1 Rat)
    Tags
    10xHis tag, Avi tag, MBP, and muGFP
    Expression
    Bacterial
    Mutation
    V208M, C273S, C391S and C393S (over NTS1-B5)
    Promoter
    Lac/T5
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    199152-plasmid-map-sequence-id-393468

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  9. PRDX6A-c001

    Plasmid
    #175474
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX6 (PRDX6 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    See Depositor Comments
    Available since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175474-plasmid-map-sequence-id-353175

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  11. pET22b-EC12WTC9AAvi

    Plasmid
    #47925
    Purpose
    Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.
    Depositor
    Tanja Kortemme
    Publication
    Ritterson et al J Am Chem Soc. 2013 Aug 7.
    Insert
    E-Cadherin EC12
    Tags
    6xHis, Avi Tag, and TEV Cleavage
    Expression
    Bacterial
    Mutation
    changed Cysteine 9 to Alanine
    Promoter
    T7
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47925-plasmid-map-sequence-id-68712

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  14. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid
    #112712
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells
    Depositor
    Daniel Schoenberg
    Publication
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    EGFP
    Tags
    HIV Rev nuclear localization signal (NES), biotin…
    Expression
    Mammalian
    Mutation
    Nucleotide sequence optimized for synthetic gene …
    Promoter
    CMV with Tet repressor binding sites
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    112712-plasmid-map-sequence-id-217058

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  15. PF3D7_1431400-bio

    Plasmid
    #47791
    Purpose
    Expresses enzymatically monobiotinylated full-length PF3D7_1431400 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised PF3D7_1431400
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47791-plasmid-map-sequence-id-68871

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  16. TLP-bio

    Plasmid
    #47787
    Purpose
    Expresses enzymatically monobiotinylated full-length TLP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised TLP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47787-plasmid-map-sequence-id-68823

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  19. pTCF170 H-2L(d)

    Plasmid
    #180458
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2L(d)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180458-plasmid-map-sequence-id-355847

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Showing: 701 - 720 of 1028 results