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2Flag-pDEST-N

Plasmid

#118371

Purpose

Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRN12

Plasmid

#84456

Purpose

shuttle plasmid for sarA P1-mAmetrine expression

Depositor

Reindert Nijland

Publication

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mAmetrine

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGWB515

Plasmid

#74857

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.hygro

Plasmid

#32685

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Publication

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FU-MAP2-Gateway

Plasmid

#43915

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

V5 and non-standard His tag (HHRHHH)

Expression

Mammalian

Promoter

Human MAP2 promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pNPTS138 Cm

Plasmid

#41891

Depositor

Howard Steinman

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb

Expression

Bacterial

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pACYC-T7

Plasmid

#41187

Depositor

Dan Bolon

Publication

Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

Insert

S. cerevisiae Hsp82

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJC53.2

Plasmid

#26536

Purpose

T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Depositor

Phillip Newmark

Publication

Collins et al PLoS Biol. 2010 . 8(10):e1000509.

Type

Empty backbone

Use

T/a cloning vector for dsrna generation and the g…

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pdCas9-sgRNA-RFP

Plasmid

#166005

Purpose

Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

Depositor

Martin Buck

Publication

Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pEvol-MjaYRS

Plasmid

#153557

Purpose

Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

Depositor

Huiwang Ai

Publication

Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

Inserts

MJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter

Expression

Bacterial

Promoter

glnS and pBAD

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLENTI_RPS3_Keima

Plasmid

#127140

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSU-araC-Cas9

Plasmid

#120423

Purpose

Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

Depositor

Sheng Yang

Publication

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Promoter

araBAD

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-C

Plasmid

#118369

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDest-Sumo

Plasmid

#106980

Purpose

LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag

Depositor

Han Choe

Publication

Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10

Type

Empty backbone

Tags

His8-Sumo

Expression

Bacterial

Promoter

T7

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSEVA331Bb

Plasmid

#78269

Purpose

pSEVA331 with Biobrick multiple cloning site

Depositor

Tom Ellis

Publication

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Type

Empty backbone

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAC150-PBLHL-4xHS-EF1a-DEST

Plasmid

#48234

Purpose

PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

EF1a

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBID-UASC-VG

Plasmid

#35206

Depositor

Brian McCabe

Publication

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Type

Empty backbone

Use

Drosophila transgene expression

Tags

mVENUS

Promoter

Drosophila Synthetic Core Promoter (DSCP)

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pgLAP5

Plasmid

#19706

Depositor

Peter Jackson

Insert

pgLAP5

Tags

EGFP, Precission Protease site, and S peptide

Expression

Mammalian

Available since

Feb. 19, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pSMAL

Plasmid

#161785

Purpose

Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.

Depositor

John Dick, Peter van Galen

Publication

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and SFFV

Available since

Dec. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Flag-GFP11-pDEST-C

Plasmid

#118367

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-FLAG

Expression

Mammalian

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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