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pEC3135 (pERASE)

Plasmid

#220306

Purpose

Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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gamma Synuclein-VC

Plasmid

#89734

Purpose

expresses human gamma Synuclein fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Article

Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1

Insert

SNCG (SNCG Human)

Use

Tags

C-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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His-PaIct

Plasmid

#111293

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

succinyl-CoA:itaconate CoA transferase

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO3_F

Plasmid

#146316

Purpose

Mammalian Expression of HsAGO3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO3 (AGO3 Human)

Use

Tags

Expression

Mammalian

Mutation

T25A

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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C413-E33: attB4-UbCp>-attB1r

Plasmid

#162925

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human Ubiquitin C promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ubiquitin C promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E24: attB4-UbCp>-attB5

Plasmid

#162980

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human Ubiquitin C promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ubiquitin C promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DMRTB1

Plasmid

#222531

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMRTB1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMRTB1 (DMRTB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HOXA5

Plasmid

#222538

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXA5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXA5 (HOXA5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DMRTC2

Plasmid

#222548

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMRTC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMRTC2 (DMRTC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MEIOSIN

Plasmid

#222551

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MEIOSIN

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MEIOSIN (LOC388553 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DPPA3

Plasmid

#222578

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DPPA3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DPPA3 (DPPA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-CTCFL

Plasmid

#222546

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of CTCFL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

CTCFL (CTCFL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-MBP_F

Plasmid

#146318

Purpose

Mammalian Expression of MBP

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

MBP (MBP Human)

Use

Tags

Expression

Mammalian

Mutation

N217S

Promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Bmal1

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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PB-PE

Plasmid

#162796

Purpose

All-in-one prime editor piggyBac transposon

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-PLXNB2

Plasmid

#86237

Purpose

Lentivirus for expression of human Plexin-B2

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

full length PLXNB2, CRISPR resistant

Promoter

human PGK promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-RightTALE

Plasmid

#196503

Purpose

Expresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox3-FusXTBE-RightTALE

Plasmid

#196505

Purpose

Expresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_KLF4

Plasmid

#140104

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

gRNA1 under U6 and gRNA2 under H1

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pOUPc-Rh159-GFP

Plasmid

#179280

Purpose

All in one "tet-on" lentivirus vector that expresses rhesus cytomegalovirus Rh159 fused to an eGFP tag at its cytoplasmic tail. Rh159 is an ER resident protein that binds NKG2D activating ligands.

Depositor

Jeremy Kamil

Article

Zhang et al mBio. 2019 Dec 10;10(6). pii: mBio.02110

Insert

Rh159 fused to eGFP

Use

Lentiviral; All-in-one "tet" on lentivi…

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Tet Responsive Element 3G

Available since

Availability

Academic Institutions and Nonprofits only

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