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pLdCN

Plasmid

#84290

Purpose

Express gRNA and Cas9 in Leishmania with Neomycin resistance

Depositor

Greg Matlashewski

Publication

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Promoter

L. donovani ribosome RNA promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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hGip-R

Plasmid

#14942

Depositor

Bernard Thorens

Publication

Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

Insert

Gip-R (GIPR Human)

Expression

Mammalian

Mutation

Short form of the receptor

Available since

June 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
MARIO/ pcDNA3

Plasmid

#124838

Purpose

mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

Depositor

Takeharu Nagai

Publication

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

MARIO

Tags

No tag

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pOpen-ETSSB

Plasmid

#165549

Purpose

ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Extreme Thermostable Single-Stranded DNA Binding Protein

Use

Synthetic Biology

Available since

Aug. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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NLS pEGFP-N3 (1299)

Plasmid

#62043

Purpose

mammalian expression of NLS-EGFP

Depositor

Eric Schirmer

Publication

Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

Insert

SV40 NLS

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pM-CBE

Plasmid

#196293

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding CBE in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

Plasmid

#64112

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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p5069 pcDNA4c hBrd4 (471-730)

Plasmid

#14448

Depositor

Peter Howley

Publication

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (471-730) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 471-730

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pCDH-EF1-FHC-POLQ-DM

Plasmid

#64880

Purpose

lentiviral expression of human POLQ double mutant (in POL and HDL domains)

Depositor

Richard Wood

Publication

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K121M and D2330A,Y2331A

Promoter

EF1alpha

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-140

Plasmid

#118556

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLPC 12S E1A

Plasmid

#18740

Depositor

Scott Lowe

Publication

Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pFB2E

Plasmid

#195288

Purpose

Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Publication

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-GFP-HYGRO

Plasmid

#115144

Purpose

Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Publication

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

GFP

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5 Fyn deltaSH3

Plasmid

#16034

Depositor

Filippo Giancotti

Publication

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

Delta SH3: deletion of amino acids 76-141

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pQUAST-FLPo

Plasmid

#24357

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

FLPo

Expression

Insect

Available since

April 12, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMH5

Plasmid

#52531

Purpose

expresses FLP recombinase in Drosophila

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

FLP recombinase (flp Budding Yeast)

Expression

Insect

Promoter

ubiquitin

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HA-ISceID44A

Plasmid

#59424

Purpose

Expresses a catalytically inactive form of the endonuclease ISceI

Depositor

Tom Misteli

Publication

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

endonuclease ISceI mutant (I-SceI )

Tags

HA tag

Expression

Mammalian

Mutation

changed aspartate 44 to alanine (D44A)

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDH-EF1-FHC-POLQ-DY2230AA

Plasmid

#64878

Purpose

lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

Depositor

Richard Wood

Publication

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

D2330A,Y2331A, in the DNA polymerase domain (POL)…

Promoter

EF1alpha

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCFJ353 - MCS(ttTi4391, I)

Plasmid

#34869

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4391 targeting

Use

Worm targeting

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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LSL

Plasmid

#66590

Purpose

Lox-STOP-Lox reporter

Depositor

Wen Xue

Publication

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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