We narrowed to 1,232 results for: sumo

1. MZB224

   Plasmid

   #226998

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   T31N

   Promoter

   T7

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

   Plasmid

   #221447

   Purpose

   Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   SPR degron: hGEM (1-110) fragment that is fused t…

   Promoter

   CMV

   Available since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA5-FRT-TO-EGFP-N

   Plasmid

   #210270

   Purpose

   N-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Type

   Empty backbone

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 RPS6KL1 MIT, residues 20-140

   Plasmid

   #180623

   Purpose

   Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   RPS6KL1 (RPS6KL1 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 20-140

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 SNX15 MIT residues 263-342

   Plasmid

   #180625

   Purpose

   Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SNX15 (SNX15 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 263-342

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA528 SPASTIN , F124D, residues 108-200

   Plasmid

   #180629

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200, F124D

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 SPASTIN , L177D, residues 108-200

   Plasmid

   #180634

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200, L177D

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 USP8, residues 1-147

   Plasmid

   #180636

   Purpose

   Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   USP8 (USP8 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-147

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA528 CALPAIN-7 residues 1-165

   Plasmid

   #180611

   Purpose

   Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-165

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCA528 CALPAIN-7 residues 1-165, V18D

    Plasmid

    #180612

    Purpose

    Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165, V18D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCA528 CALPAIN-7 residues 1-165, F98D

    Plasmid

    #180613

    Purpose

    Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-165, F98D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. hHSF4b K294R

    Plasmid

    #118357

    Purpose

    This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K294R

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gal4-HSF4b WT

    Plasmid

    #118358

    Purpose

    This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDB24

    Plasmid

    #91959

    Purpose

    empty expression vector for 10His-SUMO tag protein purification

    Depositor

    Christopher Bahl

    Type

    Empty backbone

    Use

    Tags

    10-His and Smt3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDG04135

    Plasmid

    #196013

    Purpose

    Nanobody Zasp-Nano65 production

    Depositor

    Dirk Görlich

    Insert

    H14-SUMO-Cys- ZaspNano65-Cys
    

    Use

    E.coli expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDG04136

    Plasmid

    #196014

    Purpose

    Nanobody Zasp-Nano66 production

    Depositor

    Dirk Görlich

    Insert

    H14-SUMO-Cys- ZaspNano66-Cys
    

    Use

    E.coli expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDG04095

    Plasmid

    #196006

    Purpose

    Nanobody Obs-Nano56 production

    Depositor

    Dirk Görlich

    Insert

    H14-SUMO-Cys- ObsNano56-Cys
    

    Use

    E.coli expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDG03247

    Plasmid

    #195993

    Purpose

    Nanobody Sls-Nano42 production

    Depositor

    Dirk Görlich

    Insert

    H14-SUMO-Cys- SlsNano42-Cys
    

    Use

    E.coli expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDG04092

    Plasmid

    #196009

    Purpose

    Nanobody Obs-Nano59 production

    Depositor

    Dirk Görlich

    Insert

    H14-SUMO-Cys- ObsNano59-Cys
    

    Use

    E.coli expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDG04110

    Plasmid

    #196012

    Purpose

    Nanobody Actn-Nano64 production

    Depositor

    Dirk Görlich

    Insert

    H14-SUMO-Cys- ActnNano64-Cys
    

    Use

    E.coli expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge