Addgene

We narrowed to 16,452 results for: EMB

Showing: 7181 - 7200 of 16452 results

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pMK446 (BSD-P2A-BromoTag-mClover)

Plasmid

#214376

Purpose

BromoTag-mClover for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag-mClover

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK447 (BSD-P2A-BromoTag-mCherry)

Plasmid

#214377

Purpose

BromoTag-mCherry for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag-mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK466 (mAB-BSD)

Plasmid

#214392

Purpose

mAID-BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

mAID-BromoTag-BSD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha

Plasmid

#179713

Purpose

Lentiviral expression of mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pXNubWtgate

Plasmid

#105080

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Use

Tags

NubI

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1C389S

Plasmid

#108128

Purpose

Recruitable inactive SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1L(C389S)

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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TAL-L1-1.3-Ctrl

Plasmid

#102355

Purpose

Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

Depositor

Maria-Elena Torres-Padilla

Article

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-1.3

Use

T3 in vitro transcription for expression in mamma…

Tags

FLAG and HA

Expression

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn spCas9

Plasmid

#179725

Purpose

human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

spCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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PIGAZFN-L1

Plasmid

#37797

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PIGA zinc finger L1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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CuGFP-ATG8(416)

Plasmid

#49423

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

Depositor

Daniel Klionsky

Article

Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

CUP1

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pTK162_mCherry-4.1G-hardened

Plasmid

#46361

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Modified for siRNA resistance to gcTccTcaTttCgaAc…

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g10

Plasmid

#80793

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g9

Plasmid

#80792

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g8

Plasmid

#80791

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pSin wPGK-Cre

Plasmid

#101242

Purpose

low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Cre recombinase

Use

Lentiviral

Tags

Expression

Mutation

see attached file

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pKL1-222

Plasmid

#219409

Purpose

Nuclear expression of Synrophin PDZ clamp protein

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

pTEF1-NLS-(Syntrophin-PDZ)x4-tADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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Expression scaffold for dCas9+sgRNA

Plasmid

#171799

Purpose

PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

Depositor

Stephen Elledge

Article

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Insert

PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P11

Plasmid

#120022

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pQE80l A-CC-A

Plasmid

#196478

Purpose

Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

Depositor

Fei Sun

Article

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

A-CC-A

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2a

Plasmid

#157816

Purpose

Fluorescent reporter for histone H2A tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H2A (H2ac13 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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