We narrowed to 67,891 results for: TOR

1. AAV-hSyn-RiboL1-jGCaMP8f

   Plasmid

   #169249

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. [pSK24] pACYCDuet His-c7orf59|HBXIP

   Plasmid

   #136143

   Purpose

   bacterial expression of c7orf59 and HBXIP

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

   Insert

   c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)
   

   Expression

   Bacterial

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. hu-TLR4 cDNA wt pDEST40

   Plasmid

   #42646

   Purpose

   destination vector for expression of human TLR4 in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 (TLR4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

   Plasmid

   #162388

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCh-BICD2*-strep

   Plasmid

   #120168

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   BICD cargo adaptor 2 (Bicd2 Mouse)
   

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated BICD2: 15-595 aa

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5 WT KSR1

   Plasmid

   #25970

   Depositor

   Rob Lewis

   Article

   Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

   Insert

   Kinase suppressor of Ras 1 (Ksr1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-M4-AR-S81A

   Plasmid

   #171241

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)

   Depositor

   Steven Balk

   Article

   Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

   Insert

   3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Ser81Ala (AR-S81A)

   Promoter

   CMV

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTet-O-LHX8-T2A-PuroR

   Plasmid

   #162350

   Purpose

   Lentiviral expression of LHX8 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   LHX8 (LHX8 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Promoter

   Tet-ON

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. INS-2A-luciferase-2A-CD19-GFP

   Plasmid

   #159349

   Purpose

   Human INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Inserts

   Luciferase-2A-human_CD19-2A-GFP
   Luciferase-2A-human_CD19-2A-GFP (CD19 Human)
   

   Use

   Ins locus knockin donor vector

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA-10Xcapture-PuroR

    Plasmid

    #211496

    Purpose

    Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

    Depositor

    Lesley Forrester, Antonella Fidanza

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    U6-gRNA
    Ef1a-PuroR
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a and U6

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiV_Neo_POU2F3_CR

    Plasmid

    #122175

    Purpose

    Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Insert

    POU2F3 (POU2F3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    3 silent mutations for gRNA resistance (882g-c, 8…

    Promoter

    EFS

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4c/His-Xpress-HA-hBIG1(E793K)

    Plasmid

    #79433

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG1 (ARFGEF1 Human)
    

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 793 to Lysine

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162383

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SGyA

    Plasmid

    #160292

    Purpose

    Drives expression of Cas9 and a TdTomato fluorescent marker

    Depositor

    Omar Akbari

    Article

    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

    Inserts

    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    

    Tags

    T2A GFP

    Expression

    Insect

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162387

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CAG-ChR2-YFP

    Plasmid

    #204345

    Purpose

    PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    ChR2-YFP
    

    Tags

    Yellow fluorescent protein

    Expression

    Mammalian

    Mutation

    C-terminus (aa315-737) is deleted - PMID: 1461559…

    Promoter

    CAG

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIneo-AR-S256A

    Plasmid

    #171227

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser256Ala (AR-S256A)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser256Ala mutant (AR Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser256Ala (AR-S256A)

    Promoter

    CMV

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIneo-AR-S308A

    Plasmid

    #171229

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser308Ala (AR-S308A)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser308Ala mutant (AR Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser308Ala (AR-S308A)

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIneo-AR-S90A

    Plasmid

    #171225

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser90Ala mutant (AR Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser90Ala (AR-S90A)

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208041

    Purpose

    Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    TurboID
    

    Use

    Lentiviral

    Promoter

    EFS

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. TFRC-His-bio

    Plasmid

    #52338

    Purpose

    Expresses full-length Transferrin receptor protein 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TFRC (TFRC Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. Tet-O-FUW-EGFP-P2A-mCherry

    Plasmid

    #203868

    Purpose

    Tet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGFP
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCEP4-myc-PDGFRalpha

    Plasmid

    #136434

    Purpose

    Mammalian expression plasmid for c-myc-tagged PDGFRA

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBS-AMVpl-δWT-nAchR

    Plasmid

    #97188

    Purpose

    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    Delta nicotinic acetylcholine receptor (Chrnd Mouse)
    

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBS-AMVpl-γWT-nAchR

    Plasmid

    #97187

    Purpose

    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    Gamma nicotinic acetylcholine receptor (Chrng Mouse)
    

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. Opto-M1R

    Plasmid

    #106069

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-M1R (CHRM1 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. GLuc-PM-Venus

    Plasmid

    #164785

    Purpose

    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Gaussia princeps, Human)
    

    Tags

    GLuc and mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. Opto-GPR146

    Plasmid

    #106047

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR146; Opto-GPR146; E1)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR146 (GPR146 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDYT002

    Plasmid

    #186550

    Purpose

    Lineage tracing vector (with barcoded Target Sites and triple sgRNAs

    Depositor

    Jonathan Weissman

    Article

    Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

    Insert

    14-bp random integration barcode and three target sites
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162384

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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