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ARB351

Plasmid

#124034

Purpose

Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mRuby2

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L2-C3_CAG-Lyn::mRuby2-SV40_RE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcFN-C (chick fibronectin)

Plasmid

#14063

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin (FN1 Chicken)

Expression

Mammalian

Mutation

DraI - EcoRI fragment inserted in HincII-EcoRI si…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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MTK0_013

Plasmid

#123928

Purpose

Encodes the phiC31 attB GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Phic31attB destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_030

Plasmid

#123692

Purpose

Encodes ConL1 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1-P2A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MSP3.6-bio-His

Plasmid

#50804

Purpose

Expresses enzymatically monobiotinylated full length MSP3.6

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSP3.6

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PFB0475c-bio-His

Plasmid

#50822

Purpose

Expresses enzymatically monobiotinylated full length PFB0475c

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFB0475c

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z04

Plasmid

#120047

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P30

Plasmid

#120041

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_050

Plasmid

#123947

Purpose

Encodes the hCLYBL landing pad destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hCLYBL landing pad destination vector

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8b_011

Plasmid

#123898

Purpose

Encodes the 3' 500 bp hAAVS1 locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4b_001

Plasmid

#123840

Purpose

Encodes Bovine Growth Hormone polyadenylation signal as a Type 4b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Bgh pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8a_004

Plasmid

#123888

Purpose

Encodes the F1 Ampicillin resistance cassette as a type 8a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-AmpR-ColE1

Expression

Mammalian

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8_004

Plasmid

#123884

Purpose

Encodes the F1 Ampicillin resistance cassette as a type 8 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-AmpR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8b_001

Plasmid

#123890

Purpose

Encodes the 5' PiggyBac transposon recognition site cassette as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

5'PB

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK7_001

Plasmid

#123870

Purpose

Encodes the 3' PiggyBac transposon recognition site cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3'PB

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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CALCOCO1 V142A-mycDDK

Plasmid

#137768

Purpose

CALCOCO1 CLIR mutant

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 V142A

Tags

mycDDK

Expression

Mammalian

Mutation

V142A

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3_016

Plasmid

#123731

Purpose

Encodes Gs-coupled rM3D as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

rM3D(Gs)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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mGDF8 donor mCherry-PA

Plasmid

#72596

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

mCherry-puromycin cassetter

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR U6 L1-R5

Plasmid

#62111

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-TAL-NC2

Plasmid

#43855

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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