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pAV0663

Plasmid

#133478

Purpose

Expression in fission yeast from promoter pact1, integrated at his5 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-NatMx

Plasmid

#122897

Purpose

ADE2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pNR284

Plasmid

#79550

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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BASIC_2_Kan-MB1

Plasmid

#68136

Purpose

storage vector for BASIC part: Kan-MB1

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

Kan-MB1

Expression

Bacterial

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pBLIC-puro

Plasmid

#45197

Purpose

a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.

Depositor

Priyamvada Rai

Article

Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only
pOTTC155

Plasmid

#50949

Purpose

An LV packaging vector expressing eGFP

Depositor

Brandon Harvey

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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rGH-ABC

Plasmid

#32618

Depositor

Connie Cepko

Article

Billings et al PLoS One. 2010 . 5(10):e13739.

Insert

rGH-ABC

Expression

Mammalian

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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rGH-PAL

Plasmid

#32616

Depositor

Connie Cepko

Article

Billings et al PLoS One. 2010 . 5(10):e13739.

Insert

rGH-PAL

Expression

Mammalian

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ1594

Plasmid

#11413

Depositor

Sung-Hou Kim

Article

Wang et al Structure. 2001 Jan 10. 9(1):65-71.

Insert

phosphoserine phosphatase (MJ_1594 M. jannaschii)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pIM04

Plasmid

#132584

Purpose

pZS2-pLsoxS-TEVp-proD-soxR

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

pLsoxS-TEVp-TermT1
proD-soxR-TermT1

Expression

Bacterial

Promoter

pLsoxS and proD

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pIM16

Plasmid

#132583

Purpose

pZA4-oxySp-mCherry-pLsoxS-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-mCherry-TermT1
pLsoxS-mCherry-TermT1

Expression

Bacterial

Promoter

oxySp and pLsoxS

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pG20_Venus_Blp

Plasmid

#159704

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Tags

mVenus

Expression

Plant

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pIM09

Plasmid

#132582

Purpose

pZE1-oxySp-sfGFP-proD-oxyR

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-sfGFP-TermT1
proD-oxyR-TermT1

Expression

Bacterial

Promoter

oxySp and proD

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pIM23

Plasmid

#132586

Purpose

pZA4-oxySp-mCherry-TEVrs-LAA

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-mCherry-TEVrs-LAA-TermT1
None

Expression

Bacterial

Promoter

none and oxySp

Available Since

July 17, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_WT_GFP (ABE8e_V28C_nCas9_WT)

Plasmid

#251548

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx1 V28C

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231346

Purpose

Self complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nmEML-mRFP

Plasmid

#231178

Purpose

Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCW-12S-E1A-HA-puro

Plasmid

#212671

Purpose

Express adenovirus E1A

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

12S-E1A

Use

Lentiviral

Tags

HA

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Lepr peak reporter-eGFP

Plasmid

#196584

Purpose

Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

eGFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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