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pMC-1-ccdB-6

Plasmid

#197811

Purpose

Level 0 J1-J6 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-ccdB-9

Plasmid

#197812

Purpose

Level 0 J6-J9 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-ccdB-11

Plasmid

#197813

Purpose

Level 0 J9-J11 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRetrosuper USP28 shRNA-1

Plasmid

#15663

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

USP28 shRNA (USP28 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pCAX-SCLM

Plasmid

#216757

Purpose

For strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoter

Depositor

George Augustine

Insert

SuperClomeleon

Expression

Mammalian

Mutation

S30R and 11 AA deletion in the Cerulean CFP moiet…

Promoter

CAG (cytomegalovirus-chicken beta-actin-rabbit be…

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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nSYB-GAL80-DD

Plasmid

#124778

Purpose

Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GAL80 (GAL80-DD)

Tags

DD

Expression

Insect

Promoter

nSynaptobrevin

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV5

Plasmid

#78629

Purpose

GESTALT V5 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V5

Use

Lentiviral

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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siSlug3

Plasmid

#10905

Depositor

Bob Weinberg

Article

Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Insert

siSlug (SNAI2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pET29b-Rhobin2

Plasmid

#239018

Purpose

Bacterial overexpression of de novo Rhodamine binder 2 (Rhobin2) protein

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

RhoBin2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGGF007

Plasmid

#48847

Purpose

Provides a kanamycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::KanamycinR::tNOS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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FIRESFLIP-PBCS-E670

Plasmid

#228701

Purpose

Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAC-BETAipi-ispA/dxs

Plasmid

#178302

Purpose

Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

ispA
dxs

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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miR-124-5 promoter GFP

Plasmid

#20792

Depositor

David Bartel

Article

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-124 (mir124-5 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
TR-GFP

Plasmid

#80591

Purpose

Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAH123

Plasmid

#66077

Purpose

The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

phi80 integrase

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mScarlet

Plasmid

#191324

Purpose

Expresses fluorescent labeled beta Tubulin to measure microtubule density

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mScarlet (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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APEX2-ChBP

Plasmid

#108878

Purpose

Expression of APEX2 fused to a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-ChBP

Expression

Mammalian

Available Since

May 26, 2018

Availability

Academic Institutions and Nonprofits only

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CJ8421_04975 Rptr2 recording plasmid

Plasmid

#194211

Purpose

CJ8421_04975 Rptr2 targeted DNA sequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 Rptr2 DNA target

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJJS012

Plasmid

#188335

Purpose

PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mTagBFP2::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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