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T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4

Plasmid

#193359

Purpose

self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP-e2a-SOX2AV-p2a-KLF4 (KLF4, SOX2 Aequorea victoria, Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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CD4-C-BFP HDRT Source (pTR 176)

Plasmid

#112020

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-BFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXL2-MUT

Plasmid

#194585

Purpose

Mammalian Expression of mEGFP-FOXL2 Mutant (A328Pfs*28) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXL2 (FOXL2 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

A328Pfs*28

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-HMGB1-MUT-Patchless

Plasmid

#194563

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant "Patchless" (K184Rfs*44, "Patchless" variant) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44, truncation by stop codon after Lys209

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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CD4-C-mCherry HDRT Source (pTR 175)

Plasmid

#112019

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-mCherry HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (JN.1)

Plasmid

#241117

Purpose

Expresses SARS-CoV-2 spike (JN.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (JN.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

ins16MPLF,T19I,R21T,L24del,P25del,P26del,A27S,S50…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a-PAF1CdeltaWDR61

Plasmid

#231745

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-delFS

Plasmid

#194555

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del FS" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

truncation by stop codon after Lys183

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-Rdel

Plasmid

#194556

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R del" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Deletion of Arginines after Lys185

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-RorKtoA

Plasmid

#194558

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R or K > A" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

R or K > A substitutions after Lys185

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-DVL1-MUT

Plasmid

#194587

Purpose

Mammalian Expression of mEGFP-DVL1 Mutant (H502Pfs*143) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

DVL1 (DVL1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

H502Pfs*143

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-RtoA

Plasmid

#194557

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > A" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

R > A substitutions after Lys185

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP-e2a-SOX2-p2a-KLF4

Plasmid

#193358

Purpose

VEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP-e2a-SOX2-p2a-KLF4 (KLF4, SOX2 Aequorea victoria, Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_BiCHATe27_Gq_dTomato

Plasmid

#213832

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe4_Gq_dTomato

Plasmid

#213946

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiVIPe4_ChR2_mCherry

Plasmid

#213856

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe4_eGFP

Plasmid

#221463

Purpose

AAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

eGFP

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-EML4-ALK variant 3a G1202R

Plasmid

#183830

Purpose

Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells

Depositor

Aaron Hata

Article

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-6a and ALK exon…

Expression

Mammalian

Mutation

G1202R

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EML4-ALK variant 3a L1196M/G1202R

Plasmid

#183831

Purpose

Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells

Depositor

Aaron Hata

Article

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-6a and ALK exon…

Expression

Mammalian

Mutation

L1196M/G1202R

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe4_dTomato_nlsdTomato

Plasmid

#213944

Purpose

AAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: AAV_WDS0004_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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