19,660 results

1. pCB301-2μ-HDV

   Plasmid

   #196329

   Purpose

   A yeast-Escherichia coli-Agrobacterium shuttle vector

   Depositor

   Zhenghe Li

   Article

   Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-EF1a-dCas9-VP64-Puro

   Plasmid

   #99371

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-VP64-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFG4

   Plasmid

   #87251

   Purpose

   gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Hyg
   

   Tags

   parS segments

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE puro K-Ras V12

   Plasmid

   #9052

   Purpose

   Retroviral expression of a constitutively active form of K-Ras

   Depositor

   William Hahn

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   G12V. Constitutively active.

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

   Plasmid

   #115684

   Purpose

   NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

   Depositor

   David Suter

   Article

   Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

   Insert

   Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
   

   Use

   Lentiviral

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK3_038

   Plasmid

   #123752

   Purpose

   Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   R-FlincA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT2-4xUAS:NICD-P2A-mRFP

   Plasmid

   #127594

   Purpose

   Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Insert

   NICD (notch1a Zebrafish)
   

   Use

   Zebrafish expression

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-MRLC1

   Plasmid

   #35680

   Depositor

   Tom Egelhoff

   Article

   Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

   Insert

   MRLC1 (MYL9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-Gα13*-BERKY3

   Plasmid

   #158218

   Purpose

   BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3-TLR5-CFP

    Plasmid

    #13019

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 5 (TLR5 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Flag-Tet1 CD

    Plasmid

    #70129

    Purpose

    Express flag-tagged mouse Tet1 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET1 catalytic domain.

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 Flag FKHR AAA mutant

    Plasmid

    #13508

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    FKHR AAA (FOXO1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Available Since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-Puro-BRAF-V600E

    Plasmid

    #15269

    Purpose

    Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    BRAF (BRAF Human)
    

    Use

    Retroviral

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Valine 600 to Glutamate (mutation has been found …

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRGEN-CMV-CjCas9

    Plasmid

    #89752

    Purpose

    Expression of CjCas9 in mammalian cells

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40-HA

    Expression

    Mammalian

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_CamKIIa_GFP

    Plasmid

    #96941

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Ricardo Dolmetsch, Oleksandr Shcheglovitov

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    Green fluorescent protein
    

    Use

    Lentiviral

    Promoter

    CaMKIIa

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCP20T-E2Crimson

    Plasmid

    #78473

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    E2-Crimson
    

    Expression

    Bacterial

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-RPA194

    Plasmid

    #17660

    Depositor

    Tom Misteli

    Article

    Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

    Insert

    RPA194 (Polr1a Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

18. LAMP-mCherry-CRY2

    Plasmid

    #102249

    Purpose

    Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    LAMP-mCherry-CRY2 (LAMP1, CRY2 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-hsp27 wt FL

    Plasmid

    #17444

    Depositor

    Andrea Doseff

    Article

    Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

    Insert

    small heat shock protein27 (HSPB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAd/CMV/DAX1-IRES-nEGFP

    Plasmid

    #29752

    Depositor

    Edward McCabe

    Insert

    dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
    

    Tags

    EGFP, IRES, and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    No mutations; Reporter is nuclear localized EGFP;…

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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