We narrowed to 14,249 results for: TIM
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Plasmid#128133PurposeThe cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertHipO
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95S73AGW (F2)
Plasmid#74039Purposelentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiExpressionMammalianMutationS73APromoterH1Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTN177E-myc
Plasmid#107474PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation N177E with approximately wildtype activityDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTY289I-myc
Plasmid#107480PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y289I with approximately wildtype activityDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTK37Q-myc
Plasmid#107476PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K37Q approximately wildtype activityDepositorAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH861-gst-optec P210T
Plasmid#89595PurposePlasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase P210T mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged proline 210 to threoninePromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH846-gst-dissc
Plasmid#96907PurposePlasmid for yeast expression of GST encoded by non-preferred codonsDepositorArticleInsertyeast codon dis-optimised glutathione-S transferase
Tags8xhistidineExpressionYeastPromoterTDH3Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH860-gst-optec A180Q
Plasmid#89594PurposePlasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged alanine 180 to glutaminePromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH858-gst-optec V156E
Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH859-gst-optec Y172M
Plasmid#89593PurposePlasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged tyrosine 172 to methioninePromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTL406I-myc
Plasmid#107487PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L406I with approximately wildtype activityDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTE392M-myc
Plasmid#107486PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E392M with approximately wildtype activityDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTT233F-myc
Plasmid#107481PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T233F with approximately wildtype activityDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTI576V-myc
Plasmid#107473PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576V approximately wildtype transportDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTL550V-myc
Plasmid#107472PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L550V approximately wildtype transportDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
FH95pUG+p97W (B8)
Plasmid#74017Purposelentiviral expression of Psd95 shRNA, and EGFP-SAP97beta fusionDepositorInsertPsd95 shRNA
UseLentiviral and RNAiExpressionMammalianPromoterH1Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FH95pUp97aGW (C3)
Plasmid#74019Purposelentiviral expression of Psd95 shRNA and SAP97alpha-EGFP fusionDepositorInsertPsd95 shRNA
UseLentiviral and RNAiExpressionMammalianPromoterH1Available SinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 1
Plasmid#70651PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C16orf80 sgRNA 2
Plasmid#70650PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C16orf80
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C3orf17 sgRNA 2
Plasmid#70653PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C3orf17
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_Triple
Plasmid#67733PurposeExpresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged histidine 303 to alanine, arginine 373 to…PromoterT7Available SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WTnoGirdles
Plasmid#62653PurposeWild-type OmpA with aromatic girdle residues replaced by leucineDepositorInsertOmpA_noGirdles
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationAromatic girdle residues mutated to Leu (W7, W15,…PromoterT7Available SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMA3228
Plasmid#46856PurposeA lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12DepositorInsertExoY K81M
UseLentiviralTagsFKBP E31G, R71G, K105E and MycPromoterTRE-TightAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
BDH2
Plasmid#39151PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAGI1
Plasmid#38882PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAGI1
Plasmid#38883PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAGI1
Plasmid#38901PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEG634
Plasmid#40121DepositorInsertMex-5 (aa244-468) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa244-468Promoter4.4 kb mex-5 promoter fragmentAvailable SinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95S73DGW (F3)
Plasmid#74040Purposelentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiExpressionMammalianMutationS73DPromoterH1AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARδ
Plasmid#119916PurposeMammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARδ
ExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only