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pSB61 - pL0_V2 (CDS1)

Plasmid

#123183

Purpose

Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)

Use

Part for plant expression

Tags

Expression

Plant

Mutation

No BpiI and BsaI sites

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_R89A-PolyA

Plasmid

#112291

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Human YAP1-2gamma with Arginine 89 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_L91A-PolyA

Plasmid

#112292

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Human YAP1-2gamma with Leucine 91 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_F95A-PolyA

Plasmid

#112293

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Human YAP1-2gamma with Phenylalanine 95 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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mTet3 flox donor

Plasmid

#117146

Purpose

Making Tet3 conditional knock out

Depositor

Jacob Hanna

Article

Zviran et al ssrn.3155731

Insert

Tet3 (Tet3 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Met1 diUb (1-152, R42W)

Plasmid

#110767

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

R42W

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4119-eGFP-3HA

Plasmid

#108393

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4077-3HA

Plasmid

#108392

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mPC1_4077-eGFP

Plasmid

#108391

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSN29

Plasmid

#100114

Purpose

expresses a chimeric cMYC-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-cMYC fused to full length HP1 alpha (MYC, CBX5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

Plasmid

#70667

Purpose

Human mutant of E2F1 lacking amino acids 193-359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 193 to 359 (E2F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

lacks amino acids 193-359

Promoter

cmv

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hBcl10_#16

Plasmid

#75253

Purpose

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against hBcl10 (BCL10 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hBcl10_#2

Plasmid

#75254

Purpose

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against hBcl10 (BCL10 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIX_sh_hBcl10_#1

Plasmid

#75248

Purpose

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against hBcl10 (BCL10 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

Plasmid

#70669

Purpose

Human mutant of E2F1 lacking amino acids 284 to 359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 284 to 359 (E2F1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

lacks amin acids 284 to 359

Promoter

cmv

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only
pde3b_L (OZ591)

Plasmid

#35231

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pde3b (pde3b Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pde3b_R (OZ592)

Plasmid

#35232

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pde3b (pde3b Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
LOC566040_ R (OZ588)

Plasmid

#35228

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting LOC566040 (LOC566040 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
fam82a2_L (OZ573)

Plasmid

#35213

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting fam82a2 (rmdn3 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
LOC566040_ L (OZ587)

Plasmid

#35227

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting LOC566040 (LOC566040 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only