We narrowed to 12,721 results for: CAR

1. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231364

   Purpose

   Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAnc80L65

   Plasmid

   #68837

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   Ancestral AAV Capsid Anc80L65
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Rep2

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO-TRE-AFOS

   Plasmid

   #197079

   Purpose

   A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

   Depositor

   Elaine Fuchs

   Article

   Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

   Insert

   AFOS (Fos Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and T7

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EFIa-Id1-PGK-puro

   Plasmid

   #107735

   Purpose

   Expresses Id1 in mammalian cells

   Depositor

   Alexandre Colas

   Article

   Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

   Insert

   Id1 (T Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BSD-P2A-TagRFP-hSmad1-donor

   Plasmid

   #203662

   Purpose

   Donor plasmid for CRISPR knock-in

   Depositor

   Ali Hemmati Brivanlou

   Article

   Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

   Insert

   smad1 (SMAD1 Human)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-ChrimsonR-tdT

   Plasmid

   #59169

   Purpose

   Expression vector for ChrimsonR-tdTomato

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   K176R

   Promoter

   CAG

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. TCR012H

   Plasmid

   #233454

   Purpose

   Expresses ncHLAp-reactive T cell receptor (TCR012H)

   Depositor

   William Freed-Pastor

   Article

   Ely et al Science. 2025. May 8; 388(6747)

   Insert

   TCR012H
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3- SREBP-1

   Plasmid

   #204192

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Use

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   multiple silent mutations

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJET-CMV-GFP-P2A-K20-P2A-mCherry

   Plasmid

   #185618

   Purpose

   Ribosomal stalling reporter: positive control

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   GFP-K20(AAA)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJET-CMV-GFP-P2A-K0-P2A-mCherry

    Plasmid

    #185619

    Purpose

    Ribosomal stalling reporter: empty

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-empty-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJET-CMV-GFP-P2A-I15-P2A-mCherry

    Plasmid

    #185620

    Purpose

    Ribosomal stalling reporter: 15x isoleucine

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-I15-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAX-EGFP-FBP17

    Plasmid

    #178365

    Purpose

    Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Formin Binding Protein 17 (FNBP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRM055 His-TEV-Cas9 6xNLS 0C

    Plasmid

    #196246

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-GABRA1(A322D)-IRES2-EGFP

    Plasmid

    #170821

    Purpose

    human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

    Depositor

    Tingwei Mu

    Article

    Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

    Insert

    Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    change Alanine 322 to Aspartate

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR4 CDS A. thaliana PHOT1

    Plasmid

    #209187

    Purpose

    pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    AT3G45780.1 (PHOT1 Mustard Weed)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Rab35 WT

    Plasmid

    #47424

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 (RAB35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES2-hPAC-FLAG

    Plasmid

    #129494

    Purpose

    Expresses human TMEM206 tagged at the C-terminus with FLAG

    Depositor

    Zhaozhu Qiu

    Article

    Yang et al Science. 2019 Apr 26;364(6438):395-399.

    Insert

    human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

    Plasmid

    #163169

    Purpose

    Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    Non-targeting
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKII-pAce-Kv2.1PR

    Plasmid

    #195529

    Purpose

    Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAce-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 78K, 81D, 92N, 178F; SY linker

    Promoter

    CaMKII

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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