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pRL1058

Plasmid

#70686

Purpose

Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

Depositor

Peter Wolk

Publication

Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

Insert

Transposon Tn5-1058

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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Pr-delRBS-deGFP

Plasmid

#67744

Purpose

Pr promoter expressing a deGFP that is missing a ribosomal binding site

Depositor

Richard Murray

Publication

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3-K83,88,104A

Plasmid

#58405

Purpose

Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysines 83, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83A

Plasmid

#58401

Purpose

Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 83 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24A

Plasmid

#58400

Purpose

Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 24 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pWzl_neo_DEST_flag_BFP

Plasmid

#45302

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Retroviral

Tags

flag

Expression

Mammalian

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_LUC

Plasmid

#45290

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_ARPM1

Plasmid

#45259

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

ARPM1 (ACTRT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_SAMD7

Plasmid

#45275

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SAMD7 (SAMD7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt344A

Plasmid

#34601

Depositor

Michael Ruppert

Publication

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-344 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt206B

Plasmid

#34600

Depositor

Michael Ruppert

Publication

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-206 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
Ki67p-FUCCI-2K7 Bsd

Plasmid

#255891

Purpose

Used to differentiate between quiescent and dividing cells and track progression through the cell cycle

Depositor

Alex Zambon

Publication

Zambon et al Cytometry A. 2010 Jun;77(6):564-70. doi:

Insert

FUCCI reporters

Use

Lentiviral

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAVEC2_Empty

Plasmid

#243801

Purpose

pAVEC2 backbone with no fluorophores or tags

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Agrobacterium mediated transformation

Expression

Plant

Promoter

35S

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAVEC1_Empty

Plasmid

#243800

Purpose

pAVEC1 backbone with no fluorophores or tags

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Agrobacterium mediated transformation

Expression

Plant

Promoter

35S

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-miRFP703-dSal-p53NT(1-97)-S15D-hCRY2-NLS

Plasmid

#241842

Purpose

Expresses an actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Publication

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

N-terminus (1-97 aa) containing S15D

Promoter

CAGGS promoter

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pAVEC0_Empty

Plasmid

#243799

Purpose

pAVEC0 backbone with no fluorophores or tags

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Agrobacterium mediated transformation

Expression

Plant

Promoter

35S

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CIBN-p53CT(98-393)-mNeonGreen

Plasmid

#241844

Purpose

Expresses a localizer of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Publication

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CIBN and mNeonGreen

Expression

Mammalian

Mutation

C-terminus (97-393 aa)

Promoter

CAGGS promoter

Available since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-miRFP703-dSal-VP16minADx3-hCRY2-NLS

Plasmid

#241843

Purpose

Expresses an actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Publication

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

VP16

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

VP16 minimal activation domain x 3

Promoter

CAGGS promoter

Available since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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ISA861: Melezitose pathway

Plasmid

#241971

Purpose

Melezitose pathway phagemid. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Publication

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

melezitose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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ISA179: Tagatose pathway

Plasmid

#241968

Purpose

Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Publication

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

Tagatose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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