We narrowed to 11,806 results for: nsf

1. pAAV MCS mCherry-KASH

   Plasmid

   #139654

   Purpose

   Cloning template for AAV-based ORANGE knock-in constructs

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-BRX-eGFP-NLS

   Plasmid

   #217534

   Purpose

   AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   BMP reporter element and miniXon casette (Smad1 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   BMP Reporter Element

   Available Since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV sgRNA Expression Plasmid

   Plasmid

   #174540

   Purpose

   Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

   Depositor

   Ormond MacDougald

   Article

   Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Tags

   None

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE347

   Plasmid

   #153228

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. v1em-Cterm-PE2max-U6-pegRNA

   Plasmid

   #198733

   Purpose

   AAV genome encoding C-terminal PE2max and U6 expression cassette

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   NpuC-CtermPE2max
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. v1em-Nterm-PE2max-U6-sgRNA

   Plasmid

   #198732

   Purpose

   AAV genome encoding N-terminal PE2max and U6 expression cassette

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   Nterm PE2max-NpuN
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS_sfGPF 150TAG

   Plasmid

   #154765

   Purpose

   plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. OmpA-mNG-sfTq2ox

   Plasmid

   #124220

   Purpose

   Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

   Depositor

   Tanneke den Blaauwen

   Article

   Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

   Insert

   OmpA-mNG-sfTq2ox
   

   Expression

   Bacterial

   Available Since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-Electra2-P2A-EGFP

   Plasmid

   #184936

   Purpose

   Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   Electra2-P2A-EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-EGFP-NLS

    Plasmid

    #165441

    Purpose

    Expresses nuclear EGFP from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    EGFP-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-4mtGCaMP6f-DIO

    Plasmid

    #179529

    Purpose

    Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

    Depositor

    Maarten Kole

    Article

    Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

    Insert

    4mtGCaMP6f
    

    Use

    AAV

    Tags

    4x Cox8 targeting sequence

    Promoter

    Ef1a

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-fDIO-mNeonGreen

    Plasmid

    #99133

    Purpose

    An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    fDIO-mNeonGreen
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGM65881

    Plasmid

    #153215

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-eYFP

    Plasmid

    #104055

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

    Insert

    EYFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

    Plasmid

    #166055

    Purpose

    Expresses the full length TurboID on the surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.hSyn.CaMPARI.WPRE.SV40

    Plasmid

    #100832

    Purpose

    AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Loren Looger, Eric Schreiter

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    CaMPARI
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-Electra1-P2A-EGFP

    Plasmid

    #184935

    Purpose

    Expresses blue fluorescent protein Electra1 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-P2A-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEFBOS_CreIRESpuro

    Plasmid

    #183812

    Purpose

    Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

    Depositor

    Ed Stanley

    Article

    Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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