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pCk4_spacer

Plasmid

#136074

Purpose

Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk2

Plasmid

#136696

Purpose

Acceptor plasmid for odd level position 2, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-MCS-3AID-HA-2A-mCherryBSD

Plasmid

#225700

Purpose

Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

T2A-mCherry

Expression

Mammalian

Promoter

pEF1A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p160Tol2-her4.3:switchCas9

Plasmid

#227680

Purpose

Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

loxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and her4.3

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt7

Plasmid

#184508

Purpose

Mammalian expression of Synaptotagmin 7 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 7 (Syt7 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Cav-RA

Plasmid

#209881

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

CAVIN1

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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HSP70-pp7i-mCh-UPG

Plasmid

#161011

Purpose

C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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jk68

Plasmid

#87020

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB-4xλN(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pVH1002-Tier2(pUC ori)

Plasmid

#169600

Purpose

Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCk1

Plasmid

#136695

Purpose

Acceptor plasmid for odd level position 1, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-NAD

Plasmid

#193821

Purpose

CMV overexpression of ChemoG-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-NAD

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCk4

Plasmid

#136698

Purpose

Acceptor plasmid for odd level position 4, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-NICD1

Plasmid

#46048

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Entry vector

Tags

Myc tag

Mutation

Cytoplasmic domain initiating at amino acid 1753

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pCMV MDM-2

Plasmid

#16441

Depositor

Bert Vogelstein

Article

Oliner et al Nature. 1992 Jul 2. 358(6381):80-3.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174529

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Inserts

PylS
PylT

Tags

Flag

Expression

Mammalian

Promoter

U6

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPERretro-Sirt7 shRNA2

Plasmid

#53145

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pSUPERretro-Sirt7 shRNA1

Plasmid

#53144

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

Plasmid

#49772

Purpose

Level 2 construct

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

sgRNA_PDS2-Cas9-sgRNA_PDS1

Use

CRISPR

Expression

Plant

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Cav-B

Plasmid

#209879

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Tags

CAVIN1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6-Smad3 WT

Plasmid

#27010

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
Lv EF1a SS18-Frb PGK Puro

Plasmid

#102811

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

SS18-Frb

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

Plasmid

#69906

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (CDR1-AS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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[UBC][NRE Pum][eGFP]

Plasmid

#97192

Purpose

The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

wild type Pumilio homology domain

Use

Synthetic Biology

Tags

eGFP

Expression

Mammalian

Promoter

UBC (ubiquitin C)

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 Smad1-HA

Plasmid

#14956

Depositor

Joan Massague

Article

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) p65 shRNA1

Plasmid

#22507

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA1 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe-vHras-zeo

Plasmid

#17756

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

vHras (genomic0 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

V12D

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
E12-pCLBabe

Plasmid

#20918

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 (TCF3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G155D

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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U6-spTRE3G-CMV-mTagBFP2

Plasmid

#102854

Purpose

A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sp-TRE3G-sgRNA; mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAC1430

Plasmid

#127981

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5B-TFGbeta receptor II K277R

Plasmid

#11762

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Tags

His

Expression

Mammalian

Mutation

K277R: kinase dead

Promoter

CMV

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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