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SARS-CoV-2 spike G1345 mutant

Plasmid

#231919

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158, N1173, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G1345 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1158Q, N1173Q, N1194Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G12 mutant

Plasmid

#231914

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G12 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1134Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEXP(FLAG/HA-NSP1-WT)

Plasmid

#188781

Purpose

Expresses FLAG/HA-NSP1 in mammalian cells (SARS-CoV-2 NSP1)

Depositor

Markus Hafner

Article

Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do

Insert

FLAG/HA-NSP1 (SARS-CoV-2)

Use

Frt site to insert into hek293 t-rex genome

Tags

FLAG/HA

Expression

Mammalian

Promoter

CMV Dox inducible

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-FR-2Strep

Plasmid

#210312

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Tags

2Strep

Expression

Mammalian

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-EGFP

Plasmid

#210318

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 N-2Strep

Plasmid

#210376

Purpose

Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-COV-2 N (N SARS-CoV-2)

Tags

2Strep

Expression

Mammalian

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204797

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-nsp7

Plasmid

#201026

Purpose

Expresses SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLY237

Plasmid

#184150

Purpose

Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-R1

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY251

Plasmid

#184153

Purpose

Reprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-Ar2

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY283

Plasmid

#184156

Purpose

Reprogrammed short tracrRNA for hijacking mRNA of zntA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-zntA-1034

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[WIV1 RBD]

Plasmid

#190087

Purpose

express chimeric SARS-CoV spike with the WIV-1 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Tags

Rhodopsin C9

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[Khosta2 RBD]

Plasmid

#190083

Purpose

express chimeric SARS-CoV spike with the Khosta-2 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Tags

Rhodopsin C9

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[Khosta1 RBD]

Plasmid

#190082

Purpose

express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Tags

Rhodopsin C9

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[BtKY72 RBD]

Plasmid

#190081

Purpose

express chimeric SARS-CoV spike with the BtKY72 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Tags

Rhodopsin C9

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[PRD0038 RBD]

Plasmid

#190085

Purpose

express chimeric SARS-CoV spike with the PRD0038 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Tags

Rhodopsin C9

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Clover4-LUMABS-S1 RBD

Plasmid

#183491

Purpose

To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-S1 RBD

Expression

Insect

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb21

Plasmid

#184679

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb21 (S Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTF

Plasmid

#162784

Purpose

Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

Depositor

Ivan Vorobiev

Article

Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

Type

Empty backbone

Tags

10xHis and FLAG

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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