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pSR357.9

Plasmid

#125105

Purpose

Expression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)129
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.29

Plasmid

#125088

Purpose

Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)139
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.15

Plasmid

#125082

Purpose

Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoEa

Plasmid

#121900

Purpose

Plasmid for making zebrafish apoEa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoEa (apoea Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGI vpx

Plasmid

#115815

Purpose

Encodes codon optimized 3xFLAG-SIVAGI Vpx (HM803690) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGI Vpx (HM803690)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-DRL vpx

Plasmid

#115814

Purpose

Encodes codon optimized 3xFLAG-SIVDRL Vpx (KM378563) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDRL Vpx (KM378563)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MNE vpx

Plasmid

#115813

Purpose

Encodes codon optimized 3xFLAG-SIVMNE Vpx (U79412) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMNE Vpx (U79412)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pM1GG

Plasmid

#108200

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

M. sedula PCNA1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF6-1/1

Plasmid

#91515

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF6-1/1 (ARHGEF6 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SKAP2-1/1

Plasmid

#91447

Purpose

Protein expression and purification of human SH3 domain construct SKAP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SKAP2-1/1 (SKAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FYB-1/2

Plasmid

#91459

Purpose

Protein expression and purification of human SH3 domain construct FYB-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FYB-1/2 (FYB1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FYB-2/2

Plasmid

#91300

Purpose

Protein expression and purification of human SH3 domain construct FYB-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FYB-2/2 (FYB1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-chCARex

Plasmid

#87233

Purpose

bacterial expression of GST-CAR extracellular domain

Depositor

Fritz Rathjen

Article

Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

Insert

CXADR (CXADR Chicken)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pPromE8 (GB0914)

Plasmid

#68175

Purpose

Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTNos:BASTA:PNos (GB0023)

Plasmid

#68211

Purpose

Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

bar gene

Use

Unspecified

Mutation

BsaI and BsmBI sites removed

Promoter

PNos

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pP2A11 (GB0082)

Plasmid

#68168

Purpose

Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pJW4.17

Plasmid

#62872

Purpose

Bacterial expression of MBP-MBK-2 K196R

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2 K196R

Tags

MBP

Expression

Bacterial

Mutation

K196R

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only
pSARSF53-259

Plasmid

#61757

Purpose

NpuDnaB(delta283)dC39 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

NpuDnaB(delta283)dC39 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRAmut-polyA

Plasmid

#59782

Purpose

This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Expression

Mammalian

Mutation

Change from 'GATCGAAGTTTG' to 'GAc…

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal W427A

Plasmid

#47349

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

W427A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only