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Plasmid#158992PurposeMulticolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV hSyn hPGK1-mRuby
Plasmid#220911PurposeNeuronal specific expression of the fusion construct PGK1-mRubyDepositorInsertPGK1-mRuby (PGK1 Human)
UseAAVTagsmRubyExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CARD8-TEV-GFP-FLAG
Plasmid#169991PurposeMammalian purification construct for CARD8DepositorInsertCARD8 (CARD8 Human)
UseTagsTEV-GFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-PGK-eGFP
Plasmid#193023PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (PGK) promoterDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALIX(1-702)-mNeonGreen
Plasmid#191193PurposeTruncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.DepositorInsertALIX (1-702) (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationDeletion of ALIX C-terminal PRD.PromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321G
Plasmid#98670PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321GDepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationA321GPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MuHKII-pGFPN3
Plasmid#21922DepositorInsertMutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the full length human HKII cDNA sequence …PromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBE2QL1-Strep/HA
Plasmid#124666PurposeExpresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cellsDepositorInsertUBE2QL1 (UBE2QL1 Human)
UseTags2xStrep/HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
SiC-V2-Cas9G7
Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-STChRger2-TS-EYFP
Plasmid#129393PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertsoma targeted ChRger2
UseAAVTagsKv2.1-TS-EYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sema4g-Fc-His
Plasmid#72160PurposeExpresses the extracellular region of the Sema4G protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema4g (Sema4g Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
UseTagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2_SEPT9 i4
Plasmid#71617Purposemammalian expression of human SEPT9 i4 with a GFP fusionDepositorInsertSeptin 9 (SEPTIN9 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX-Z4 (GB3375)
Plasmid#160648PurposepLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-HA3-HPS1
Plasmid#133930PurposeExpresses 3xHA-HPS1 construct in mammalian cellsDepositorInsertHPS1 (HPS1 Human)
UseTags3xHA tagExpressionMammalianMutationPromoterCMV I.E.Available sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 BARK1 minigene
Plasmid#14695DepositorInsertBARK1 minigene (GRK2 Bovine)
UseTags3'UTR of human b-globinExpressionMammalianMutationBARK1 amino acids 495-689PromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 Flag Jip1b
Plasmid#52123Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.a(L)-Fc-His
Plasmid#72151PurposeExpresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3g.a (Sema3g Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMVhPOP1_3Flag
Plasmid#53968PurposeMammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.DepositorInsertPOP1 (POP1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p38KTRClover
Plasmid#59142PurposeORF encoding p38 KTR mClover flanked by Gateway sequencesDepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only