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pAAV-hSYN1-NES-OCaMP-WPRE

Plasmid

#224934

Purpose

pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide

Depositor

Alexander Lohman

Publication

Aggarwal et al Nat Commun. 2026 Jun 22. doi: 10.1038/s4

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSynI

Available since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDisplay-GRAB-HaloDA1.0-IRES-EGFP-CAAX

Plasmid

#234410

Purpose

Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Publication

Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

Insert

GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0

Expression

Mammalian

Promoter

CMV

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CoV2-Omicron BA.1 spike

Plasmid

#194602

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

Depositor

Ben Murrell

Publication

Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

Insert

CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Publication

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

Plasmid

#163572

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A T31A-dCas9

Plasmid

#163537

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-dCas9

Use

CRISPR

Expression

Mammalian

Mutation

dCas9 (D10A, H840A)eA3A T31A

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

Plasmid

#163545

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Publication

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLux02

Plasmid

#78282

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Publication

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pBullet-er-c

Plasmid

#53068

Purpose

destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Publication

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 9

Plasmid

#36107

Depositor

Narasimhan Gautam

Publication

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 9

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
mcherry Beta 4

Plasmid

#36114

Depositor

Narasimhan Gautam

Publication

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B4 (Gnb4 Mouse)

Tags

mcherry

Expression

Mammalian

Promoter

cmv

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
punc-119c

Plasmid

#21595

Depositor

Al Fisher

Publication

Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

Insert

unc-119 (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Expression

Worm

Mutation

N/A

Available since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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myc-Rapr-FAK-YM

Plasmid

#25927

Depositor

Klaus Hahn

Publication

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

This myc-RapR-FAK-YM plasmid contains FAK mutatio…

Available since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCas12a-NT

Plasmid

#235167

Purpose

Expresses the Wild Type Francisella novicida cas12a gene and a NT crRNA

Depositor

Constantinos Patinios

Publication

Della Valle et al Trends Biotechnol. 2025 Jun;43(6):1470-1

Insert

FnCas12a

Expression

Bacterial

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-uTEV3

Plasmid

#234320

Purpose

Mammalian expression of uTEV3 tagged with an eGFP marker

Depositor

Mateo Sánchez

Publication

Silva-Rojas et al Nat Biomed Eng. 2025 Jun 5. doi: 10.1038

Insert

eGFP-uTEV3

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Mutations respective to the wild type TEV: S219V …

Promoter

CMV

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pHPHS800

Plasmid

#228240

Purpose

Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

Depositor

Arvind Rasi Subramaniam

Publication

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Insert

Cas9

Tags

Hygromycin phosphotransferase

Expression

Mammalian

Promoter

none

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E9

Plasmid

#213472

Purpose

Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing

Depositor

Yiping Qi

Publication

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadDE-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tadde-32aa linker-zspc…

Tags

NO

Expression

Plant

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-EGFP

Plasmid

#166140

Purpose

EGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

EGFP

Expression

Yeast

Promoter

GAL1

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Plasmid

#218795

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Publication

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Use

AAV

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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