We narrowed to 7,572 results for: Lif

1. pHR-BRD4ΔN-mCherry-sspB

   Plasmid

   #121968

   Purpose

   Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-461

   Promoter

   SFFV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-Flag-Med25

   Plasmid

   #64771

   Purpose

   Mammalian protein expression of flag-tagged Med25

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25 (MED25 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pUCmini-iCAP-PHP.eC

   Plasmid

   #201903

   Purpose

   non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

   Insert

   Synthetic construct isolate AAV-PHP.eC VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCmini-iCAP-PHP.V1

   Plasmid

   #127847

   Purpose

   non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.V1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF6-CSPG4-myc-his

   Plasmid

   #69037

   Purpose

   Expression of CSPG4 in mammalian cells

   Depositor

   Wensheng Wei

   Article

   Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

   Insert

   CSPG4 (CSPG4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1 alpha

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT-TODestFLAGHAhFXR1

   Plasmid

   #48694

   Purpose

   Expresses hFXR1

   Depositor

   Thomas Tuschl

   Article

   Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

   Insert

   FlagHA FXR1 (isoform b) (FXR1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P2-MDM2 (17-125)

   Plasmid

   #62063

   Purpose

   Expression of human MDM2 (17-125) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 17-125

   Promoter

   tac

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P2-p53 (1-90) (72R)

   Plasmid

   #62081

   Purpose

   Expression of human p53 (TAD residues 1-90) (72R) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains TAD/PP residues 1-90, 72R

   Promoter

   tac

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pUCmini-iCAP-PHP.N

   Plasmid

   #127851

   Purpose

   non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.N VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT-TODestFLAGHAhFMRPiso1

    Plasmid

    #48690

    Purpose

    Expresses hFMRP iso 1

    Depositor

    Thomas Tuschl

    Article

    Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

    Insert

    FlagHA FMR1 Iso 1 (FMR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid

    #49077

    Purpose

    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x HA and mCerulean

    Expression

    Mammalian

    Mutation

    262 silent mutations from native hNKCC1

    Promoter

    CMV

    Available since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-CMV-Flag-PGC1a-6His

    Plasmid

    #67637

    Purpose

    AAV vector expressing PGC1a gene

    Depositor

    Connie Cepko

    Article

    Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

    Insert

    PGC1a (Ppargc1a Mouse)
    

    Use

    AAV

    Tags

    6XHis and Flag tag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 Flag YFP hNKCC1 (NT17)

    Plasmid

    #49085

    Purpose

    Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    262 silent mutations from native hNKCC1

    Promoter

    CMV

    Available since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid

    #55613

    Purpose

    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Silent mutations have been incorporated into the …

    Promoter

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR26-CMVconst-mBMP7

    Plasmid

    #127375

    Purpose

    Allows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Insert

    BMP7 (Bmp7 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV enhancer/promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UBC-S1-GCaMP6f

    Plasmid

    #160722

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cav2.1 HA pcDNA3

    Plasmid

    #206069

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR-53BP1 1-1972 WT

    Plasmid

    #53446

    Purpose

    Recombinational donor/master vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    siRNA resistant and mutations A128V, T720A, A1347…

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pSUMO His6 SUMO SnRK2.6 (11-362)

    Plasmid

    #177847

    Purpose

    Bacterial Expression of SnRK2.6

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.6 (OST1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-UBC-S2-cAMPr

    Plasmid

    #160723

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-cAMPr
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge