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pCAFNF-LifeAct-EGFP

Plasmid

#163608

Purpose

Expresses LifeAct fused to EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

LifeAct

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD113

Plasmid

#172959

Purpose

pRMCD77 encoding Wzz mutant G305A/G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G311A mutant gene

Expression

Bacterial

Mutation

G305A/G311A, A208V - please see depositor comments

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-124

Plasmid

#171558

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-124

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-124

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCL27-decoder

Plasmid

#159449

Purpose

Insert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1tet-citrine-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1tet

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMM725

Plasmid

#133594

Purpose

Part Plasmid for Linus-Mxi, Part 4a(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

Linus-Mxi1

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM727

Plasmid

#133596

Purpose

Part Plasmid for Mxi1, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

Mxi1

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM582

Plasmid

#133590

Purpose

Part Plasmid for Linus, Part 4a(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

Linus

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM728

Plasmid

#133597

Purpose

Part Plasmid for Mxi1, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

Mxi1

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMM726

Plasmid

#133595

Purpose

Part Plasmid for Mxi1, Part 3a(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

Mxi1

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRC03

Plasmid

#114682

Purpose

DHFR-sfGFP.Strand11

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

DHFR-sfGFP.Strand11

Tags

GST

Promoter

T7 Promoter

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-eIF4E-S200C

Plasmid

#37229

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

eIF4E

Expression

Bacterial

Mutation

S200C

Promoter

T7

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
pTYB2-eIF4B-A130C

Plasmid

#37227

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF3

Expression

Bacterial

Mutation

A130C

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pUC19-DAD4

Plasmid

#37224

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

DAD4 (DAD4 Budding Yeast)

Expression

Bacterial

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pMT FRB-Draper INT-mCherry

Plasmid

#126666

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain mCherry

Expression

Insect

Availability

Academic Institutions and Nonprofits only

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pLVX_Venus

Plasmid

#247758

Purpose

Lentiviral expression of Venus in mammalian cells

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

Venus

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX_ECFP

Plasmid

#247757

Purpose

Lentiviral expression of ECFP in mammalian cells

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

ECFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-Aurora A

Plasmid

#198402

Purpose

Expression of EGFP-tagged Aurora A in mammalian cells

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

Aurora A

Tags

EGFP

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.32_LV-mCherry-MS2x8-BCS

Plasmid

#205539

Purpose

Express mCherry-MS2x8 with barcode

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2WT

Plasmid

#101221

Purpose

Blue-light dependent optogenetic protein Cry2

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2PHR

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE-tdTomato

Plasmid

#50798

Purpose

Expression of tdTomato under the control of the TRE-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

TREtight driven tdTomato fluorescent protein

Use

Mutli-species

Promoter

TREtight

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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