We narrowed to 8,596 results for: Set

1. p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM

   Plasmid

   #183150

   Purpose

   Expression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)

   Depositor

   Assaf Mosquna

   Article

   Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

   Inserts

   Lex-ER-B112
   Zif268 DBD - hPRLBD - MSN2AD
   

   Use

   For yeast genomic integration

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-GFP-AT

   Plasmid

   #133306

   Purpose

   "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   axe/txe toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDC-UAS-nBitbow1.0(miniW)

   Plasmid

   #138329

   Purpose

   Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.

   Depositor

   Dawen Cai

   Article

   Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3

   Inserts

   mAmetrine
   tdKatushka2
   mNeonGreen
   mTFP1
   mKusabira-Orange2
   

   Tags

   human H2B

   Expression

   Insect

   Promoter

   10xUAS

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-cBEST

   Plasmid

   #125689

   Purpose

   C to T base editor for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

   Insert

   streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   ermE*/tipA

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDonor-GDH

   Plasmid

   #140629

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   Mini-Tn6677, T7lac-GDH gene cassette
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211845

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   6xUAS-35Smini and pUBQ10

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-B/c

   Plasmid

   #227962

   Purpose

   Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Promoter

   None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBV65

   Plasmid

   #108363

   Purpose

   A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

   Depositor

   Scott Moye-Rowley

   Article

   Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

   Insert

   loxP-natMX6-CgMET3prom-cre-loxP
   

   Use

   Gene disruption marker for candida glabrata

   Promoter

   C. glabrata MET3

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pv6_TAF3_2xPhd

   Plasmid

   #179399

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   TAF3_2xPhd
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDM068

    Plasmid

    #216811

    Purpose

    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Type

    Empty backbone

    Use

    CRISPR; Fungal expression

    Expression

    Bacterial

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-flex-MCS--4x6T

    Plasmid

    #196420

    Purpose

    Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pv6_CBX7_2xChromo

    Plasmid

    #179396

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_2xChromo
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    G113V in flexible linker

    Promoter

    CAGGS

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Neo-CAG-Flpe-ERT2

    Plasmid

    #68460

    Purpose

    AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    mammalian-codon-optimized Flpe recombinase fused with ERT2
    

    Use

    Synthetic Biology

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDual-IN-SMN1

    Plasmid

    #215721

    Purpose

    dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN1 EXON 7 (SMN1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR-Cre stuffer v4

    Plasmid

    #158033

    Purpose

    Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPFO*

    Plasmid

    #120400

    Purpose

    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

    Depositor

    Arun Radhakrishnan

    Article

    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

    Insert

    Mutant form of Perfringolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Amino acids 1-27 are deleted, starts at amino aci…

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMDC32B-AtMIR390a-B/c

    Plasmid

    #51776

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtMIR390a-B/c
    

    Use

    Plant expression

    Mutation

    A. thaliana MIR390a precursor sequence including …

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

    Plasmid

    #86421

    Purpose

    N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    CDC42pr-KAR2SS-GFP1-10
    

    Tags

    GFP1-10

    Expression

    Yeast

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pv6_MBD1_MBD

    Plasmid

    #179404

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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