We narrowed to 9,089 results for: Set
-
Plasmid#153229PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 LAMP1-mNeonGreen HRD
Plasmid#207549PurposeHomologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cellsDepositorInsertTET inducible LAMP1-mNeonGreen
TagsmNeonGreenExpressionMammalianPromoterEndogenousAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
EC2_4_dCas9_VPR_sgRNA
Plasmid#163709PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1-Lck-GCaMP2
Plasmid#24794DepositorInsertLck-GCaMP2
TagsHis and XpressExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…Available SinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2Aneo v2
Plasmid#80033PurposeUsed to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trappingDepositorTypeEmpty backboneUseAAV and Cre/Lox; A platform vector to construct t…Available SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F2
Plasmid#184164PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAT71
Plasmid#204305PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRPromoterie1Available SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272
Plasmid#154834PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanerceptDepositorInsertsETN
ETN
UseCre/LoxExpressionMammalianPromoter100RPU.2Available SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMX-DivisionRecorder
Plasmid#179446PurposeRetroviral expression vector containing the DivisionRecorder cassetteDepositorInsertGFP-P2A-511-CrePI-511
UseRetroviralPromoterLTRAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGE1109
Plasmid#202773PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRExpressionPlantAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOCC305
Plasmid#118898Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and TwinStrep-MB…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE666
Plasmid#153231PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM55285
Plasmid#153212PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH4
Plasmid#168980PurposeAMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATmin-GG
Plasmid#191352PurposeClostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV92.2
Plasmid#78409PurposepKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron constructDepositorInsertsRT-Cas1
Cas2
Available SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETcLIC-GFP
Plasmid#183514PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coliDepositorTypeEmpty backboneTags10x His-tag and folding reporter GFPExpressionBacterialPromoterT7 lacAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS8_7569_v1
Plasmid#126865Purpose7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubationDepositorInsertIV_CS8_7569_v1
UseSynthetic BiologyAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only