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pBABE YAP1

Plasmid

#15682

Depositor

Joan Brugge

Article

Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-his-BACH1 WT

Plasmid

#17642

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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AAV hSyn hPGK1-mRuby

Plasmid

#220911

Purpose

Neuronal specific expression of the fusion construct PGK1-mRuby

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-mRuby (PGK1 Human)

Use

AAV

Tags

mRuby

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab7L1-c-Myc

Plasmid

#79803

Purpose

Express TagBFP labeled human Rab7L1.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab7L1 (RAB29 Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pET28-His-TEV-NQO1

Plasmid

#119058

Purpose

For expression in E.coli and affinity purification of N-terminally His tagged NQO1

Depositor

Yosef Shaul

Article

Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

6His, TEV site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab5a-c-Myc

Plasmid

#79801

Purpose

Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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PB533_2E12LI-sfGFP

Plasmid

#167527

Purpose

H3K27me3-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Tjalsma et al EMBO Rep. 2021 Feb 19:e51989. doi: 10.15

Insert

2E12LI-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-DARPin-E11

Plasmid

#226784

Purpose

Expression of His-tagged DARPin E11 in E. coli

Depositor

Sebastian Mathea

Article

Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

Insert

DARPin E11

Tags

FLAG and His

Expression

Bacterial

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-R-iLACCO1

Plasmid

#208025

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

Plasmid

#233080

Purpose

Expression vector for sgRNA-S1b and eCas12f1i

Depositor

Kyoungmi Kim

Article

Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

Insert

U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP

Use

CRISPR

Tags

2xFLAG

Expression

Mammalian

Promoter

hU6, CBh

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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Amber-free pNL4-3 FLuc Env-RT-

Plasmid

#238415

Purpose

An amber-free HIV NL4-3 carrying a Firefly luciferase reporter gene, RT and Env impaired.

Depositor

Maolin Lu

Article

Xu et al STAR Protoc. 2025 Jul 26;6(3):103995. do

Insert

Firefly luciferase (LOC116160065 Synthetic)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Alternation of amber stop codons "TAG" …

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_HcRed

Plasmid

#25892

Purpose

Gateway entry vector containing HcRed.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

hcRed

Use

Gateway entry vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-deLACCO1-NGR

Plasmid

#208024

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA

Plasmid

#176741

Purpose

Expresses membrane bound biotin ligase, BioID2, in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-13xLinker-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Ezr-BioID2-HA

Plasmid

#176744

Purpose

Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Ezr-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRK172-6xHis-0N4R-P301S-tau-HiBiT

Plasmid

#187835

Purpose

Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

6XHis-0N4R-P301S-tau-HiBiT

Tags

6xHistidine and HiBiT

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

Plasmid

#176745

Purpose

Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Glt1-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a hMDH1v3 (+TEV)

Plasmid

#204229

Purpose

Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

hMDH1v3 (MDH1 Human)

Tags

6X His

Expression

Bacterial

Mutation

Inserted a G after ATG to maintain kozak site

Promoter

Lac

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTagBFP-C1-FKBP-PIP5K1C-HD-EE

Plasmid

#220090

Purpose

Recruitable PIP5K to make PIP2

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PIP5K1C (PIP5K1C Human)

Tags

TagBFP-FKBP1A(3-108)

Expression

Mammalian

Mutation

amino acids 1-635; D101R, R304D, R445E, K446E

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Chk1-Flag

Plasmid

#22894

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Chk1 (CHEK1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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