We narrowed to 11,713 results for: NSI

1. YCe2741 HC_Kan_EF1ap_p18

   Plasmid

   #100698

   Purpose

   part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter

   Depositor

   Yizhi Cai

   Article

   Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

   Insert

   EF1a promoter (EEF1A1 Human)
   

   Use

   Synthetic Biology

   Available Since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_CASKIN2-1/1

   Plasmid

   #91261

   Purpose

   Protein expression and purification of human SH3 domain construct CASKIN2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   CASKIN2-1/1 (CASKIN2 Human)
   

   Expression

   Bacterial

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. FN(9-10, RGE)-I27-CC

   Plasmid

   #85396

   Purpose

   MTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

   Depositor

   Khalid Salaita

   Article

   Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

   Insert

   Fibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   GRGDS motif mutated to GRGES

   Promoter

   T7

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCh-1539

   Plasmid

   #82432

   Purpose

   Plasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   hs-nvGW182 chimera
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

   Promoter

   EF1A

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCh-1537

   Plasmid

   #82430

   Purpose

   Plasmid for expression of NHA-tagged human-Nematostella GW182 chimera in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   hs-nvGW182 chimera
   

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

   Promoter

   CMV

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

   Plasmid

   #70195

   Purpose

   Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   tetR-deGFP fusion
   

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMKO.1-puro/hFOXH1 shRNA6

   Plasmid

   #59310

   Purpose

   Knockdown of human FOXH1

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

   Insert

   hFOXH1 shRNA (FOXH1 Human)
   

   Use

   RNAi and Retroviral

   Promoter

   hU6

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Mouse Nanog locus 3' probe

   Plasmid

   #60037

   Purpose

   Cloned genomic fragment of murine Nanog locus used as probe for Southern Blot analysis

   Depositor

   Jacob Hanna

   Article

   Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Unspecified; Cloning

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

9. TRS6E1b-luc(-732/-721) mutant 3

   Plasmid

   #45386

   Depositor

   Thomas Gelehrter

   Article

   Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

   Insert

   6 copies of hPAI-1 promoter -732/-721 mutated at -723/-721
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   -723/-721 mutated from tgt to aca

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

10. TRS6E1b-luc(-732/-721) mutant 2

    Plasmid

    #45385

    Depositor

    Thomas Gelehrter

    Article

    Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

    Insert

    6 copies of hPAI-1 promoter -732/-721 mutated at -730/-728
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    mutated at -730/-728 from aca to tgt

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pBp-FGFR2c-E471Q

    Plasmid

    #45704

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    E471Q

    Available Since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

12. BRCA1-(219-408)

    Plasmid

    #35067

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 219-408

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-hSyn-GRAB_CRF1.0

    Plasmid

    #208658

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-GRAB_SST1.0

    Plasmid

    #208665

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-GRAB_NPY1.0

    Plasmid

    #208676

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_VIP1.0

    Plasmid

    #208681

    Purpose

    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB_CCK1.0

    Plasmid

    #208673

    Purpose

    Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpySwitch

    Plasmid

    #184225

    Purpose

    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpySwitch
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-mCherry-KATNA1

    Plasmid

    #180650

    Purpose

    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GGACAGCACUCCCUUGAAA

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

    Plasmid

    #140893

    Purpose

    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel

    Depositor

    Yasushi Okamura

    Article

    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

    Insert

    CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed 223L to F in Dr-VSP (zebrafish TPTE), cha…

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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