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pSEED-OLLAS:HA

Plasmid

#219647

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

OLLAS:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-GW-GFP

Plasmid

#40123

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

GFP

Use

Retroviral

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pXD69m1-TetW

Plasmid

#191246

Purpose

E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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UPG

Plasmid

#161003

Purpose

ubiquitous expression of PCP-GFP

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PCP-GFP

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJM585 ZF6x6-C mKate2 in TUPV1

Plasmid

#161529

Purpose

Inducible expression of mKate2 under the ZF6x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

mKate2

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF6x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET30b_T7Sav

Plasmid

#138588

Purpose

T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

Depositor

Markus Jeschek

Article

Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

Insert

T7-tagged streptavidin variant with native C-terminus

Use

Synthetic Biology

Tags

T7-tag

Expression

Bacterial

Promoter

PT7

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-PKG-hNo.7

Plasmid

#175812

Purpose

The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

PKG #7

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT56

Plasmid

#204300

Purpose

Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-EGFP

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-150

Plasmid

#64235

Purpose

Expresses miR-150 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR150 (MIR150 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-H50Q

Plasmid

#181724

Purpose

Galactose inducible expression of Alpha-synuclein-H50Q

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Mutation

H50Q

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-Rap2a-V5-Hygro

Plasmid

#195510

Purpose

lentiviral vector expressing RAP2A CDS with hygromycin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Hygromycin

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-LambdaN-NbALFA

Plasmid

#207627

Purpose

Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-NbALFA

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRep

Plasmid

#199599

Purpose

Expresses AAV2 rep gene; accessory plasmid for the pCMV-Cap SSM library.

Depositor

George Church

Article

Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

Insert

AAV2 rep

Use

AAV

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBSFI-p110β

Plasmid

#34891

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110β (PIK3CB Human)

Use

Adaptor plasmid

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

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Setd3-Flag-pcDNA3.0

Plasmid

#49144

Purpose

mammalian expression of Flag tagged Setd3

Depositor

Jing H Wang

Article

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only
pRRL-VinculinCS

Plasmid

#200309

Purpose

Lentivirus vector expressing vinculin with FRET conformational sensor

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

VinculinCS

Use

Lentiviral

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMGA-ptet-sfGFP

Plasmid

#139935

Purpose

aTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

Depositor

Christopher Voigt

Article

Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

Insert

TetR-Ptet-sfGFP

Expression

Bacterial

Available Since

July 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe puro MTH1

Plasmid

#21295

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
CMVTCRa_candidate10

Plasmid

#165007

Purpose

Expression of candidate TCR #10 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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