We narrowed to 1,652 results for: RCA

Showing: 1081 - 1110 of 1652 results

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TDP-43 _CTD_5A→M_IDR

Plasmid

#194267

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human, Synthetic)

Tags

6XHistag

Expression

Bacterial

Mutation

A297M, A367M, A381M, A388M, A391M mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-SNAP-CD4-bla

Plasmid

#179452

Purpose

Donor vector to knock in SNAPtag C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 Erg (317-486)

Plasmid

#66992

Purpose

Bacterial expression plasmid for mouse Erg (317-486) with GST

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (317-486) (Erg Mouse)

Tags

GST fusion

Expression

Bacterial

Mutation

Amino acids from 317-486

Promoter

TAC

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T1 Erg (209-316)

Plasmid

#66991

Purpose

Bacterial expression plasmid for mouse Erg (209-316) with GST

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (209-316) (Erg Mouse)

Tags

GST fusion

Expression

Bacterial

Mutation

Amino acids from 209-316

Promoter

TAC

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
PX458-sgTSC2

Plasmid

#128098

Purpose

CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

sgRNA against human TSC2 (TSC2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Bmal1

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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mCRY1 HA tag

Plasmid

#110298

Purpose

full length wt mouse Cry1 HA tag in pcDNA3.1

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

wt mcry1 HA tag (Cry1 Mouse)

Tags

HA tag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-his-BACH1 K52R

Plasmid

#17643

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

The lysine residue at position 52 was changed to …

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
pDONR223_VHL_WT

Plasmid

#81874

Purpose

Gateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107010

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-WT

Plasmid

#128580

Purpose

For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107009

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Brg1 Del2 pET28a

Plasmid

#122116

Purpose

Expression Brg1 amino acids 1-837 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

6XHis and His-T7

Expression

Bacterial

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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nAChR alpha4 YFP

Plasmid

#15245

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

alpha4-YFP-M (Chrna4 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
pQCXIH BRGI-Flag

Plasmid

#19148

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
Brg1 Del3 pET28a

Plasmid

#122117

Purpose

Expression Brg1 amino acids 836-1446 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIPZ Myc2-mouse TTP aa S52 S178 MUT

Plasmid

#107001

Purpose

Expresses epitope-tagged double-mutant murine TTP

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

tristetraprolin (Zfp36 Mouse)

Tags

Double Myc peptide epitope tag

Expression

Mammalian

Mutation

Changed Ser52 to Ala and Ser178 to Ala

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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nAChR beta2 YFP

Plasmid

#15107

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

beta2-YFP-M (Chrnb2 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+ Flag-RAP2A

Plasmid

#128099

Purpose

Expresses RAP2A in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

RAP2A (RAP2A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-Luciferase-hvTK-bla

Plasmid

#179450

Purpose

Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

Luciferase

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc-his-BACH1 P47A

Plasmid

#17644

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

To generate the tumor-associated mutant, the prol…

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
hTph2p-Luc

Plasmid

#223526

Purpose

Firefly-luciferase reporter expression driven by human Tph2 promoter

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Tph2 promoter (TPH2 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

hTph2 promoter

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-mClover3-CD4-bla

Plasmid

#179448

Purpose

Donor vector to knock in mClover3 N-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mClover3

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #1

Plasmid

#179454

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #2

Plasmid

#179455

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCrisprV2 sgRNA hPER2c-term #3

Plasmid

#179456

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a RUVBL2 Delete

Plasmid

#159143

Purpose

Expression and protien purification of a mutated RUVBL2

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL2 (RUVBL2 Human)

Expression

Bacterial

Mutation

amino acid 2-456, Thr127-Glu233 mutated to GPPG

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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