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Showing: 1441 - 1480 of 11031 results

  1. pVZ_PcoaT_SQE1_LUP1

    Plasmid
    #103857
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    lupeol synthase 1 (LUP1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103857-plasmid-map-sequence-id-217123

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  13. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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  17. pPHR-mCherry-FUSN-mHP1alpha

    Plasmid
    #181892
    Purpose
    Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181892-plasmid-map-sequence-id-359691

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  19. pPHR-mCherry-nE-mHP1alpha

    Plasmid
    #181890
    Purpose
    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-nE-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    S11E, S12E, S13E, S14E
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181890-plasmid-map-sequence-id-359697

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  20. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

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  21. pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

    Plasmid
    #181893
    Purpose
    Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181893-plasmid-map-sequence-id-359709

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  22. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid
    #181891
    Purpose
    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181891-plasmid-map-sequence-id-359705

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  27. p5059

    Plasmid
    #198154
    Purpose
    bacterial expression of steC::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsteC steC::L16::HaloTag::HA
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    198154-plasmid-map-sequence-id-386983

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  28. pAC32 [TU#839]

    Plasmid
    #16079
    Depositor
    Martin Chalfie
    Insert
    mec-2 intron 9
    Use
    Tags
    YFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 23, 2008
    Availability
    Academic Institutions and Nonprofits only
    16079-plasmid-map-sequence-id-8472

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  29. pME-QF2

    Plasmid
    #83307
    Purpose
    codes for QF2 (truncated QF) in a gateway middle entry backbone.
    Depositor
    Marnie Halpern
    Insert
    QF
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    83307-plasmid-map-sequence-id-173284

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  30. p4305

    Plasmid
    #198150
    Purpose
    bacterial expression of sifA::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198150-plasmid-map-sequence-id-388083

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  31. p4286

    Plasmid
    #198152
    Purpose
    bacterial expression of sseJ::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsseJ sseJ::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198152-plasmid-map-sequence-id-388084

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  32. p4118

    Plasmid
    #198151
    Purpose
    bacterial expression of sseF::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsseA::sscB sseF::L16::HaloTag::HA (sseF Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    198151-plasmid-map-sequence-id-388048

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  33. p4295

    Plasmid
    #198153
    Purpose
    bacterial expression of pipB2::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PpipB2 pipB2::L16::HaloTag::HA (STM2780 Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198153-plasmid-map-sequence-id-388088

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  34. pTRIPZ (M)-HT-NLS

    Plasmid
    #82518
    Purpose
    Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Nuclear localizaition sequence
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82518-plasmid-map-sequence-id-156794

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  35. AtFRD3

    Plasmid
    #117086
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    AtFRD3
    Use
    Tags
    His
    Expression
    Yeast
    Mutation
    Promoter
    AOX
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117086-plasmid-map-sequence-id-224949

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  36. PX458_TGIF2_1

    Plasmid
    #72374
    Purpose
    Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    TGIF2 (TGIF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72374-plasmid-map-sequence-id-134714

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  37. PX458_TGIF2_2

    Plasmid
    #72375
    Purpose
    Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    TGIF2 (TGIF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72375-plasmid-map-sequence-id-132125

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  38. p6173

    Plasmid
    #198157
    Purpose
    bacterial expression of sopD2::HaloTag fusion, mutant form
    Depositor
    Michael Hensel
    Insert
    PsopD2 sopD2 deltaCC::L16::HaloTag::HA (STM0972 Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198157-plasmid-map-sequence-id-387011

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  39. p6172

    Plasmid
    #198156
    Purpose
    bacterial expression of pipB2::HaloTag fusion, mutant form
    Depositor
    Michael Hensel
    Insert
    PpipB2 pipB2 deltaCC::L16::HaloTag::HA (STM2780 Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198156-plasmid-map-sequence-id-388057

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  40. p6203

    Plasmid
    #198159
    Purpose
    bacterial expression of sseJ::HaloTag fusion, mutant form
    Depositor
    Michael Hensel
    Insert
    PsseJ sseJ deltaCC::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    198159-plasmid-map-sequence-id-388030

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Showing: 1441 - 1480 of 11031 results